EFFECTS OF EQUOL ON MITOCHONDRIAL HOMEOSTASIS DISORDER INDUCED BY PALMTIC ACID IN L6 CELLS
Objective To investigate the effects of equol(Eq)on palmitic acid(PA)-induced mitochondrial homeostasis in L6 cells,and to explore the related mechanisms.Methods L6 cells were cultured in DMEM(containing 10%FBS)and divided into control group,model group,PA+Eq group,PA+Eq+SR18292 group,PA+Eq+Phttp group and PA+Eq+RM02812 group.The levels of reactive oxygen species,cell viability,and glucose uptake ability at different times were measured.Additionally,changes of mitochondrial membrane potential and autophagy intensity after 24 hours of intervention were examined.We used fluorescence to label the mitochondria and observed the morphological changes of mitochondria.Transmission electron microscopy was used to observe mitochondrial morphology and autophagosome.The proteins of PINK1,PARKIN,P62,LC3Ⅰ,LC3Ⅱ,MFN2,DRP1,and PGC-1ɑ were detected by Westen blotting.Results After treatment with 0.25 mmol/L PA for 6 h,ROS level was significantly increased(P<0.05),cell viability was decreased(P<0.05),and glucose intake was decreased(P<0.05).Compared with the model group,Eq could significantly reduce ROS level,increase cell viability and glucose consumption(P<0.05).After 24 hours of PA exposure,mitochondrial membrane potential and autophagy level were significantly decreased(P<0.05).Compared with the model group,Eq could improve the decrease of mitochondrial membrane potential,promote mitochondrial fusion,and reduce mitochondrial fission(P<0.05).The protein expression of Parkin,PINK1,P62,LC3Ⅱ,MFN2 and PGC-1ɑ was significantly increased(P<0.05),and the expression of DRP1 protein was significantly decreased(P<0.05).Conclusion Eq can improve mitochondrial homeostasis disorder induced by palmitic acid in L6 cells.Eq reduces mitochondrial fission,which may be partially related to the promotion of mitophagy.[ACTA NUTRIMENTA SINICA,2024,46(2):163-170]
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