QUERCETIN INTERVENTION IMPROVES GUT MICRO-ENVIRONMENT IN MICE TREATED WITH ANTIBIOTICS A DOSE-RESPONSE STUDY
Objective To investigate the effects of different doses of quercetin on gut microbiota in mice treated with antibiotics,and explore effective dose of quercetin.Methods Sixty male Kunming mice were randomly divided into control group(C),model group(M,0 mg/kg quercetin),low dose quercetin group(LQ,200 mg/kg),moderate dose quercetin group(MQ,400 mg/kg),and high dose quercetin group(HQ,600 mg/kg).The gut microbial disorder was induced by gavage of an antibiotic cocktail.The C and M groups were fed the AIN-93G diet,and LQ,MQ,HQ groups were fed the AIN-93G diets with different doses of quercetin for 10 days.At the end of experiment,the fecal,small intestinal tissue,blood samples were collected.16SrDNA high-throughput sequencing,gas chromatography,hematoxylin-eosin staining and ELISA methods were used to analyze gut microbial structure,short chain fatty acids,intestinal tissue and gut permeability,respectively.Results Compared with the C group at phylum level,7 days antibiotic cocktail treatment decreased gut microbial diversity,except Proteobacteria,Firmicutes,and Bacteroidetes,which indicate gut microbial disrupted model were successfully established.Compared with the M group,LQ,MQ,HQ groups decreased Firmicutes/Bacteroides ratio by 32.8%、66.3%、62.9%,respectively.At the genus level,the inhibitory rates of different doses of quercetin on Escherichia Shigella and Klebsiella were all up to 99%.The increased bacteria genus Lactococcus and Desulfovibrio in the LQ group were positively related with butyric acid content(correlation coefficients were 0.74 and 0.78,respectively,P＜0.001).The fecal butyric acid content in the LQ group((0.27±0.12)mmol/kg)was significantly higher than in the M group((0.04±0.02)mmol/kg,P＜0.05)).In addition,the recovery of intestinal mucosa and villi became more pronounced with the increase of quercetin dosage.The content of diamine oxidase in serum decreased by 29.25%,37.90%,and 41.49%,respectively.Conclusion Lower quercetin dose was beneficial for butyric acid producing bacteria growth,while moderate and high quercetin doses were effective in inhibiting conditional pathogenic bacteria,and stabilizing intestinal micro-environment.[ACTA NUTRIMENTA SINICA,2024,46(4):356-364]
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