首页|A Novel Poly(3-hexylthiophene)Engineered Interface for Electrochemical Monitoring of Ascorbic Acid During the Occurrence of Glutamate-Induced Brain Cytotoxic Edemas

A Novel Poly(3-hexylthiophene)Engineered Interface for Electrochemical Monitoring of Ascorbic Acid During the Occurrence of Glutamate-Induced Brain Cytotoxic Edemas

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Although neuroelectrochemical sensing technology offers unique benefits for neuroscience research,its application is limited by substantial interference in complex brain environments while ensuring biosafety requirements.In this study,we introduced poly(3-hexylthiophene)(P3HT)and nitrogen-doped multiwalled carbon nanotubes(N-MWCNTs)to construct a composite membrane-modified carbon fiber microelectrode(CFME/P3HT-N-MWCNTs)for ascorbic acid(AA)detection.The microelectrode presented good linearity,selectivity,stability,antifouling,and biocompatibility and exhibited great performance for application in neuroelectrochemical sensing.Subsequently,we applied CFME/P3HT-N-MWCNTs to monitor AA release from in vitro nerve cells,ex vivo brain slices,and in vivo living rat brains and determined that glutamate can induce cell edema and AA release.We also found that glutamate activated the N-methyl-D-aspartic acid receptor,which enhanced Na+and Cl-inflow to induce osmotic stress,resulting in cytotoxic edema and ultimately AA release.This study is the first to observe the process of glutamate-induced brain cytotoxic edema with AA release and to reveal the mechanism.Our work can benefit the application of P3HT in in vivo implant microelectrode construction to monitor neurochemicals,understand the molecular basis of nervous system diseases,and discover certain biomarkers of brain diseases.

Zexuan Meng、Yuchan Zhang、Lu Yang、Shuang Zhao、Qiang Zhou、Jiajia Chen、Jiuxi Sui、Jian Wang、Lizhong Guo、Luyue Chang、Jialing He、Guixue Wang、Guangchao Zang

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Institute of Life Science,and Laboratory of Tissue and Cell Biology,Lab Teaching and Management Center,Chongqing Medical University,Chongqing 400016,China

Key Laboratory for Biorheological Science and Technology of Ministry of Education,State and Local Joint Engineering Laboratory for Vascular Implants,Bioengineering College of Chongqing University,Chongqing 400030,China

Jinfeng Laboratory,Chongqing 401329,China

Department of Pathophysiology,Chongqing Medical University,Chongqing,China

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重庆市自然科学基金JinFeng Laboratory Foundation,Chongqing,ChinaProject of Tutorial System of Medical Undergraduate in Lab Teaching and Management Center in Chongqing Medical UniversityProject of Tutorial System of Medical Undergraduate in Lab Teaching and Management Center in Chongqing Medical UniversityProject of Scientific Research and Innovative Experiment for College Student in Chongqing Medical UniversityProject of Scientific Research and Innovative Experiment for College Student in Chongqing Medical UniversityProject of Scientific Research and Innovative Experiment for College Student in Chongqing Medical UniversityNational Project of University Students Innovation and Entrepreneurship Training ProgramChongqing Municipal Education Commission Foundation

cstc2020jcyjmsxmX0330jfkyjf202203001LTMCMTS202109LTMCMTS202106SRIEP202011SRIEP202047SPIEP202167S202010631016CYS22333

2024

10.34133/research.0149

研究(英文)

研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.2024(1)
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