首页|Cold-Induced Reprogramming of Subcutaneous White Adipose Tissue Assessed by Single-Cell and Single-Nucleus RNA Sequencing

Cold-Induced Reprogramming of Subcutaneous White Adipose Tissue Assessed by Single-Cell and Single-Nucleus RNA Sequencing

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Adipose browning has demonstrated therapeutic potentials in several diseases.Here,by conducting transcriptomic profiling at the single-cell and single-nucleus resolution,we reconstituted the cellular atlas in mouse inguinal subcutaneous white adipose tissue(iWAT)at thermoneutrality or chronic cold condition.All major nonimmune cells within the iWAT,including adipose stem and progenitor cells(ASPCs),mature adipocytes,endothelial cells,Schwann cells,and smooth muscle cells,were recovered,allowing us to uncover an overall and detailed blueprint for transcriptomes and intercellular cross-talks and the dynamics during white adipose tissue brown remodeling.Our findings also unravel the existence of subpopulations in mature adipocytes,ASPCs,and endothelial cells,as well as new insights on their interconversion and reprogramming in response to cold.The adipocyte subpopulation competent of major histocompatibility complex class Ⅱ(MHCⅡ)antigen presentation is potentiated.Furthermore,a subcluster of ASPC with CD74 expression was identified as the precursor of this MHCⅡ+adipocyte.Beige adipocytes are transdifferented from preexisting lipid generating adipocytes,which exhibit developmental trajectory from de novo differentiation of amphiregulin cells(Aregs).Two distinct immune-like endothelial subpopulations are present in iWAT and are responsive to cold.Our data reveal fundamental changes during cold-evoked adipose browning.

Qing Liu、Qiaoyun Long、Jiayu Zhao、Wenjie Wu、Zexin Lin、Wei Sun、Ping Gu、Tuo Deng、Kerry Martin Loomes、Donghai Wu、Alice P.S.Kong、Jingying Zhou、Alfred S.Cheng、Hannah Xiaoyan Hui

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School of Biomedical Sciences,The Chinese University of Hong Kong,Hong Kong,China

Department of Pharmacology and Pharmacy,The University of Hong Kong,Hong Kong,China

Guangzhou Institute of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China

China-New Zealand Joint Laboratory on Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China

Department of Endocrinology,Jinling Hospital,Nanjing University,School of Medicine,Nanjing,China

National Clinical Research Center for Metabolic Diseases,and Department of Metabolism and Endocrinology,The Second Xiangya Hospital of Central South University,Changsha,China

Key Laboratory of Diabetes Immunology,Ministry of Education,and Metabolic Syndrome Research Center,The Second Xiangya Hospital of Central South University,Changsha,China

Clinical Immunology Center,The Second Xiangya Hospital of Central South University,Changsha,China

School of Biological Sciences and Maurice Wilkins Centre,University of Auckland,Auckland,New Zealand

Department of Medicine and Therapeutics,The Chinese University of Hong Kong,Hong Kong,China

Hong Kong Institute of Diabetes and Obesity,The Chinese University of Hong Kong,Prince of Wales Hospital,Shatin,Hong Kong,China

Li Ka Shing Institute of Health Sciences,The Chinese University of Hong Kong,Prince of Wales Hospital,Shatin,Hong Kong,China

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National Natural Science Foundation of China(NSFC)-Excellent Young Scientists FundHong Kong Research Grants Council General Research FundLo Kwee-Seong Biomedical Research Startup FundLo Kwee-Seong Biomedical Research Startup Fund

819220791712341971064807106481

2024

10.34133/research.0182

研究(英文)

研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.2024(1)
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