Early and Sensitive Detection of Pathogens for Public Health and Biosafety:An Example of Surveillance and Genotyping of SARS-CoV-2 in Sewage Water by Cas12a-Facilitated Portable Plasmonic Biosensor

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外文摘要：Infectious diseases severely threaten public health and global biosafety.In addition to transmission through the air,pathogenic microorganisms have also been detected in environmental liquid samples,such as sewage water.Conventional biochemical detection methodologies are time-consuming and cost-ineffective,and their detection limits hinder early diagnosis.In the present study,ultrafine plasmonic fiber probes with a diameter of 125 μm are fabricated for clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)-12a-mediated sensing of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Single-stranded DNA exposed on the fiber surface is trans-cleaved by the Cas12a enzyme to release gold nanoparticles that are immobilized onto the fiber surface,causing a sharp reduction in the surface plasmon resonance(SPR)wavelength.The proposed fiber probe is virus-specific with the limit of detection of～2,300 copies/ml,and genomic copy numbers can be reflected as shifts in wavelengths.A total of 21 sewage water samples have been examined,and the data obtained are consistent with those of quantitative polymerase chain reaction(qPCR).In addition,the Omicron variant and its mutation sites have been fast detected using S gene-specific Cas12a.This study provides an accurate and convenient approach for the real-time surveillance of microbial contamination in sewage water.

作者：

Tianzhong Li、Yuzhi Chen、Zhi Chen、Yuan Hao、Minyi Liang、Yingxia Liu、Guanyong Ou、Huanian Zhang、Yuxuan Tang、Yabing Hao、Swelm Wageh、Omar A.AI-Hartomy、Abul Kalam、Bin Zhang、Xin Shi、Xuejin Li、Han Zhang

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作者单位：

College of Physics and Optoelectronic Engineering,Institute of Translational Medicine,Department of Otolaryngology,Shenzhen Second People's Hospital,First Affiliated Hospital of Shenzhen University,Health Science Center,Shenzhen University,Shenzhen 518060,China

Shenzhen Key Laboratory of Sensor Technology,Shenzhen 518060,China

The Sixth Affiliated Hospital of Guangzhou Medical University,Qingyuan People's Hospital,Qingyuan 511518,China

Shenzhen Key Laboratory of Pathogen and Immunity,National Clinical Research Center for Infectious Disease,State Key Discipline of Infectious Disease,Shenzhen Third People's Hospital,Second Hospital Affiliated to Southern University of Science and Technology,Shenzhen 518112,China

School of Medicine,Southern University of Science and Technology,Shenzhen 518055,China

School of Physics and Optoelectronic Engineering,Shandong University of Technology,Zibo 255049,China

Department of Physics,Faculty of Science,King Abdulaziz University,Jeddah 21589,Saudi Arabia

Research Center for Advanced Materials Science(RCAMS),King Khalid University,P.O.Box9004,Abha 61413,Saudi Arabia

Department of Chemistry,College of Science,King Khalid University,P.O.Box9004,Abha 61413,Saudi Arabia

Health Sciences Institute,China Medical University,Shenyang 110000,China

The Chinese University of Hong Kong,Shenzhen 518060,China

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基金：

Instrumental Analysis Center of Shenzhen UniversityNational Natural Science Foundation of ChinaState Key Research Development Program of ChinaStabilization Support Program for Higher Education Institutions of ShenzhenShenzhen Bay Laboratory Open Fund ProjectHighend Talent Scientific Research Startup ProjectShenzhen Science and Technology R&D and Innovation Foundationfunding of King Khalid University through Research Center for Advanced Materials Science(RCAMS)

项目编号：

U1813207YS2022YFB320001120200812115548001SZBL2021080601012827-000636JCJY20200109105608771RCAMS/KKU/0010/21

出版年：

2024

DOI：

10.34133/research.0205

研究(英文)

CSTPCD

ISSN：

年,卷(期)：2024.2024(2)

参考文献量44