首页|Genetic Engineered Ultrasound-Triggered Injectable Hydrogels for Promoting Bone Reconstruction

Genetic Engineered Ultrasound-Triggered Injectable Hydrogels for Promoting Bone Reconstruction

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Genetic engineering technology can achieve specific gene therapy for a variety of diseases,but the current strategy still has some flaws,such as a complex system,single treatment,and large implantation trauma.Herein,the genetic engineering injectable hydrogels were constructed by ultrasonic technology for the first time to realize in vivo ultrasound-triggered insitu cross-linking and cell gene transfection,and finally complete insitu gene therapy to promote bone reconstruction.First,ultrasound-triggered calcium release was used to activate transglutaminase and catalyze the transamidation between fibrinogen.Simultaneously,liposome loaded with Zinc-finger E-box-binding homeobox1(ZEB1)gene plasmid(Lip-ZEB1)was combined to construct an ultrasound-triggered insitu cross-linked hydrogels that can deliver Lip-ZEB1.Second,ultrasound-triggered injectable hydrogel introduced ZEB1 gene plasmid into endothelial cell genome through Lip-ZEB1 sustained release,and then acted on the ZEB1/Notch signal pathway of cells,promoting angiogenesis and local bone reconstruction of osteoporosis through genetic engineering.Overall,this strategy provides an advanced gene delivery system through genetic engineered ultrasound-triggered injectable hydrogels.

Zhenyu Zhao、Huitong Ruan、Aopan Chen、Wei Xiong、Mingzhu Zhang、Ming Cai、Wenguo Cui

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Department of Orthopaedics,Shanghai Tenth People's Hospital,School of Medicine,Tongji University,No.301 Middle Yanchang Road,Shanghai 200072,China

Department of Orthopaedics,Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases,Shanghai Institute of Traumatology and Orthopaedics,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,197 Ruijin 2nd Road,Shanghai 200025,China

Department of Foot and Ankle Surgery,Beijing Tongren Hospital,Capital Medical University,1 Dongjiao Minxiang,Beijing 100730,China

National Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaShanghai Science and Technology CommissionShanghai Municipal Health and Family Planning CommissionShanghai Muni cipal Education Commission—Gaofeng Clinical Medicine Grant Support

2020YFA09082008227217682003658194119631002022XD05520171906

2024

10.34133/research.0221

研究(英文)

研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.2024(2)
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