首页|Age-Related Alterations in Peripheral Immune Landscape with Magnified Impact on Post-Stroke Brain

Age-Related Alterations in Peripheral Immune Landscape with Magnified Impact on Post-Stroke Brain

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Immunosenescence refers to the multifaceted and profound alterations in the immune system brought about by aging,exerting complex influences on the pathophysiological processes of diseases that manifest upon it.Using a combination of single-cell RNA sequencing,cytometry by time of flight,and various immunological assays,we investigated the characteristics of immunosenescence in the peripheral blood of aged mice and its impact on the cerebral immune environment after ischemic stroke.Our results revealed some features of immunosenescence.We observed an increase in neutrophil counts,concurrent with accelerated neutrophil aging,characterized by altered expression of aging-associated markers like CD62Land consequential changes in neutrophil-mediated immune functions.Monocytes/macrophages in aged mice exhibited enhanced antigen-presentation capabilities.T cell profiles shifted from naive to effector or memory states,with a specific rise in T helper 1 cells and T helper 17 cells subpopulations and increased regulatory T cell activation in CD4 T cells,Furthermore,regulatory CD8 T cells marked by Klra decreased with aging,while a subpopulation of exhausted-like CD8T cells expanded,retaining potent immunostimulatory and proinflammatory functions.Critically,these inherent disparities not only persisted but were further amplified within the ischemic hemispheres following stroke.In summary,our comprehensive insights into the key attributes of peripheral immunosenescence provide a vital theoretical foundation for understanding not only ischemic strokes but also other age-associated diseases.

Jianan Lu、Huaming Li、Guoqiang Zhang、Fan Yang、Xiaotao Zhang、An Ping、Zhouhan Xu、Yichen Gu、Rui Wang、Dan Ying、Jianjian Liu、Jianmin Zhang、Ligen Shi

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Department of Neurosurgery,Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou,Zhejiang,China

Clinical Research Center for Neurological Diseases of Zhejiang Province,Hangzhou,China

Brain Research Institute,Zhejiang University,Hangzhou,Zhejiang,China

Collaborative Innovation Center for Brain Science,Zhejiang University,Hangzhou,Zhejiang,China

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2024

10.34133/research.0287

研究(英文)

研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.2024(3)