首页|AZGP1P2/UBA1/RBM15 Cascade Mediates the Fate Determinations of Prostate Cancer Stem Cells and Promotes Therapeutic Effect of Docetaxel in Castration-Resistant Prostate Cancer via TPM1 m6A Modification

AZGP1P2/UBA1/RBM15 Cascade Mediates the Fate Determinations of Prostate Cancer Stem Cells and Promotes Therapeutic Effect of Docetaxel in Castration-Resistant Prostate Cancer via TPM1 m6A Modification

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Prostate cancer(PCa)is a common malignant tumor with high morbidity and mortality worldwide.The prostate cancer stem cell(PCSC)model provides novel insights into the pathogenesis of PCa and its therapeutic response.However,the roles and molecular mechanisms of specific genes in mediating fate decisions of PCSCs and carcinogenesis of PCa remain to be elusive.In this study,we have explored the expression,function,and mechanism of AZGP1P2,a pseudogene of AZGP1,in regulating the stemness and apoptosis of PCSCs and treatment resistance of docetaxel in castration-resistant prostate cancer(CRPC).We revealed that AZGP1P2 was downregulated in CRPC cell lines and PCSCs,while it was positively associated with progression-free interval.Upregulation of the AZGP1P2 enhanced the sensitivity of docetaxel treatment in CRPCs via inhibiting their stemness.RNA pull-down associated with mass spectrometry analysis,co-immunoprecipitation assay,and RNA immunoprecipitation assay demonstrated that AZGP1P2 could bind to UBA1 and RBM15 as a"writer"of methyltransferase to form a compound.UBA1,an E1 ubiquitin-activating enzyme,contributed to RBM15 protein degradation via ubiquitination modification.Methylated RNA immunoprecipitation assay displayed that RBM15 controlled the mRNA decay of TPM1 in m6A methylation.Furthermore,a xenograft mouse model and patient-derived organoids showed that the therapeutic effect of docetaxel in CRPC was increased by AZGP1P2 in vivo.Collectively,these results imply that AZGP1P2 mediates the stemness and apoptosis of PCSCs and promotes docetaxel therapeutic effect by suppressing tumor growth and metastasis via UBA1/RBM15-mediated TPM1 mRNA decay in CRPC.

Hong Wang、Ji Liu、Xiaojun Zhu、Bin Yang、Zuping He、Xudong Yao

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Department of Urology,Shanghai Tenth People's Hospital,School of Medicine,Tongji University,Shanghai,China

Urologic Cancer Institute,School of Medicine,Tongji University,Shanghai,China

Department of Urology Surgery,The Affiliated Hospital of Inner Mongolia Medical University,Hohhot,China

The Key Laboratory of Model Animals and Stem Cell Biology in Hunan Province,School of Medicine,Hunan Normal University,The Engineering Research Center of Reproduction and Translational Medicine of Hunan Province,Changsha,China

Shanghai Key Laboratory of Reproductive Medicine,Shanghai Jiao Tong University School of Medicine,Shanghai,China

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2024

10.34133/research.0252

研究(英文)

研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.2024(3)