首页|Cortical Organoid-on-a-Chip with Physiological Hypoxia for Investigating Tanshinone ⅡA-Induced Neural Differentiation

Cortical Organoid-on-a-Chip with Physiological Hypoxia for Investigating Tanshinone ⅡA-Induced Neural Differentiation

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Cortical organoids represent cutting-edge models for mimic human brain development during the early and even middle stage of pregnancy,while they often fail to recreate the complex microenvironmental factors,such as physiological hypoxia.Herein,to recapitulate fetal brain development,we propose a novel cortical organoid-on-a-chip with physiological hypoxia and further explore the effects of tanshinone ⅡA(Tan ⅡA)in neural differentiation.The microfluidic chip was designed with a micropillar array for the controlled and efficient generation of cortical organoids.With low oxygen,the generated cortical organoids could recapitulate key aspects of early-gestational human brain development.Compared to organoids in normoxic culturing condition,the promoted neurogenesis,synaptogenesis and neuronal maturation were observed in the present microsystem,suggesting the significance of physiological hypoxia in cortical development.Based on this model,we have found that Chinese herbal drug Tan ⅡA could promote neural differentiation and maturation,indicating its potential therapeutic effects on neurodevelopmental disorders as well as congenital neuropsychiatric diseases.These results indicate that the proposed biomimetic cortical organoid-on-a-chip model with physiological hypoxia can offer a promising platform to simulate prenatal environment,explore brain development,and screen natural neuroactive components.

Yue Zhi、Yujuan Zhu、Jinglin Wang、Junqi Zhao、Yuanjin Zhao

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Department of Rheumatology and Immunology,Nanjing Drum Tower Hospital,Clinical Medical College of Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing,210023,China

State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering Southeast University,Nanjing 210096,China

State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing,210096,China

Shenzhen Research Institute,Southeast University,Shenzhen,518038,China

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2024

10.34133/research.0273

研究(英文)

研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.2024(3)