首页|Protective and Adverse Roles of DDX3X in Different Cell Types in Nonalcoholic Steatohepatitis Progression

Protective and Adverse Roles of DDX3X in Different Cell Types in Nonalcoholic Steatohepatitis Progression

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Persistent hepatic cellular metabolic stress and liver inflammatory stimuli are key signatures of nonalcoholic steatohepatitis(NASH).DDX3X is a vital molecule involved in cell fate decisions in both pro-survival stress granule(SG)and pro-death NOD-like receptor family pyrin domain containing 3(NLRP3)inflammasome assembly in response to stress signals.However,the role of DDX3X in NASH remains unclear.We characterized the cell type-specific roles of DDX3X in NASH.Human liver tissues from NASH patients and normal control subjects were collected to assess DDX3X expression and distribution.Nutritional steatohepatitis models were constructed by feeding macrophage-specific DDX3X knockout(DDX3XΔMφ),hepatocyte-specific DDX3X knockout(DDX3XΔhep),and wild-type control(DDX3Xfl/fl)mice a high-fat and high-cholesterol(HFHC)diet,a methionine-and choline-deficient(MCD)diet,and a high-fat/high-iron/high-fructose/high-cholesterol,low-methionine,and choline-deficient(HFHIHFHC-MCD)diet.The study demonstrated that DDX3X was predominantly expressed in macrophages and hepatocytes in control liver tissues,and its expression was down-regulated in patients or mice with NASH.Compared to DDX3Xfl/fl littermates,DDX3XΔMφ mice showed improved liver histology in nutritional steatohepatitis models.Loss of macrophage DDX3X inhibited NLRP3 inflammasome-mediated pyroptosis,causing anti-inflammatory M2 polarization and alleviating hepatocyte steatohepatitic changes.DDX3XΔhep mice developed marked steatohepatitis in multiple nutritional steatohepatitis models compared to DDX3Xfl/fl littermates.DDX3X-deleted hepatocytes showed impaired SG assembly,leading to increased sensitivity and intolerance to metabolic stimulation and resultant steatohepatitis.In conclusion,DDX3X plays opposite roles in different cell types during the progression of NASH.A better understanding of the cell-specific differences in the crosstalk between SG formation and NLRP3 activation is crucial for developing prospective targeted DDX3X inhibitors for the treatment of NASH.

Suzhen Yang、Lin Zhou、Tianming Zhao、Hanlong Zhu、Tingting Luo、Kang Jiang、Xiaoxiao Shi、Chunyan Chen、Han Zhang、Si Zhao、Xiaoping Zou、Yuzheng Zhuge、Fangyu Wang、Lei Wang、Mingzuo Jiang、Bing Xu

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Department of Gastroenterology,the Affiliated Drum Tower Hospital of Nanjing University Medical School,Jiangsu,Nanjing,210002,China

Department of Gastroenterology,Nanjing Drum Tower Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College,Jiangsu,Nanjing,210008,China

Department of Gastroenterology and Hepatology,the Affiliated Jinling Hospital of Nanjing University Medical School,Jiangsu,Nanjing,210002,China

School of Medicine,Northwest University,Shaanxi,Xi'an,710069,China

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2024

10.34133/research.0275

研究(英文)

研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.2024(3)