摘要
采用多种色谱分离技术对楝科(Meliaceae)地黄连属(Munronia)羽状地黄连(Munronia pinnata W.Theobald)(矮陀陀)的地上部分进行化学成分研究,从其95％乙醇部分中分离得到10个化合物,并通过MS、IR、1D-和2D-NMR等谱学手段和量子化学计算方法等技术手段对其结构进行了鉴定,分别为矮陀陀脂肪酸苷A(1)、2-[6-O-((2E)-6R-羟基-2,6-二甲基.2,7.辛二烯基)-β-D-吡喃葡萄糖基]-2-甲基乳酸氧苷(2)、山奈酚-3-O-(6"-O-反式对香豆酰基)-β-D-吡喃葡萄糖苷(3)、栗苷A(4)、山奈酚-3-O-(6"-O-顺式对香豆酰基)-β-D-吡喃葡萄糖苷(5)、金色酰胺醇酯(6)、布卢门醇A(7)、蚱蜢酮(8)、curcusinol(9)和黑麦草素内脂(10).其中化合物1为新化合物,化合物2的NMR数据为首次报道.生物活性筛查显示化合物1在160 μmol/L的浓度下对HCT116、A549、HepG2、MCF7和MDAMB等肿瘤细胞均无细胞毒性作用.
Abstract
The chemical constituents of the aerial parts of Munronia pinnata W.Theobald were investigated by using chro-matographic techniques.Ten compounds were isolated from the 95％ethanol extract of Munronia pinnata.Their structures were identified as munrofatty A(1),2-[[6-O-((2E)-6R-hydroxy-2,6-dimethyl-2,7-octadienoyl)-β-D-glucopyranosyl]oxy]-2-methylpropanoic acid(2),kaempferol-3-O-(6-O-trans-p-coumaroyl)-β-D-glucopyranoside(3),castanoside A(4),kaempferol-3-O-(6"-O-cis-pcoumaroyl)-β-D-glucopyranoside(5),aurantiamide acetate(6),blumenol A(7),grasshopper ketone(8),curcusinol(9)and loliolide(10)through detailed analyses of various technical means,including MS,IR,1D-and 2D-NMR spectroscopic methods,quantum calculation methods and other technical means.Compound 1 is a new compound,while NMR data of compound 2 are presented for the first time.Compound 1 was evaluated for cytotoxicity against HCT116,A549,HepG2,MCF7 and MDAMB cells,and was non-toxic to the tested cells at a concentration of 160 μmol/L.
基金项目
广西自然科学基金(2020GXNSFBA297055)
广西科技基础条件与人才计划(桂科AA21196009)
国家重点研发计划(2022YFD1600302)
国家自然科学基金(U20A2004)
广西科学院基础研究基金(CQZ-C-1901)
桂林创新平台与人才计划(20210102-3)
广东省重点领域研究发展计划(2020B1111110003)
NETL
NSTL
万方数据