江西嗜酸链霉菌去铁胺E及金属络合物的分离鉴定与生物活性研究
Study on Isolation,Identification and Bioactivity of Desferrioxamine E and Its Metal-Complexes from Streptacidiphilus jiangxiensis
李金达 1程伯涛 2黄积武 2刘文2
作者信息
- 1. 上海师范大学化学与材料科学学院 上海 200233;中国科学院上海有机化学研究所 生命过程小分子调控全国重点实验室 上海 200032
- 2. 中国科学院上海有机化学研究所 生命过程小分子调控全国重点实验室 上海 200032
- 折叠
摘要
嗜酸链霉菌属细菌的研究主要集中在新物种的分离和鉴定上,而其次级代谢产物的研究则相对较少.从江西嗜酸链霉菌的发酵液中分离得到一个新的铝配合物铝草铵(alumioxamine,3)和两个己知化合物去铁胺E(desferrioxamine E,1)和铁草铵E(ferrioxamine E,2),并通过MS、IR、NMR以及单晶X射线衍射技术对其结构进行鉴定.从结构上来看,化合物3以铝为中心,与配体的六个O原子相连,形成六齿配合物.此外,体外细胞毒活性实验表明,在100μmol/L浓度时化合物1对4种人肿瘤细胞(Hela、PANC1、A375和MHCC-97H)均具有一定的抑制活性,细胞存活率为4.8％～66.4％.
Abstract
Research on the genus Streptacidiphilus was mainly focused on the isolation and identification of new species,with relatively less emphasis on the study of secondary metabolites.A new aluminium-complex alumioxamine(3),and two known compounds desferrioxamine E(1)and ferrioxamine E(2)were isolated from the fermentation broth of Streptacidiphi-lus jiangxiensis CGMCC 4.1857.Their structures were elucidated by MS,IR,NMR and single crystal X-ray diffraction tech-nologies.Structurally,compound 3 features aluminum(Ⅲ)as the central atom,which is bonded to six oxygen atoms of the ligand,forming a hexadentate complex.Furthermore,in vitro cytotoxicity assays showed that compound 1 exhibited potential inhibitory activity against four human tumor cell lines(Hela,PANC1,A375 and MHCC-97H)at a concentration of 100 μmol/L with cell viability ranging from 4.8％to 66.4％.
关键词
江西嗜酸链霉菌/次级代谢产物/配合物/细胞毒活性/晶体结构Key words
Streptacidiphilus jiangxiensis/secondary metabolites/complexes/cytotoxic activity/crystal structure引用本文复制引用
基金项目
科技部重大专项(2022YFC2303100)
国家自然科学基金(22193070)
国家自然科学基金(32030002)
出版年
2024
NETL
NSTL
万方数据