四苯乙烯酰胺类化合物的合成及其高灵敏度、高选择性识别Cu2+
Synthesis of Tetraphenylethylene Based Amides for Detection of Copper(Ⅱ)Ion with High Sensitivity and Selectivity
苏小龙 1李健鹏 1刘孟鑫 1邹莉 1杨得锁 1冯海涛1
作者信息
- 1. 宝鸡文理学院化学化工学院 聚集诱导发光研究中心 陕西宝鸡 721000
- 折叠
摘要
设计合成了一种四苯乙烯基酰胺类荧光探针TPE-Py,并对其结构进行了表征.通过荧光发射光谱和HeLa细胞成像实验研究了其金属离子识别性能.结果表明:探针TPE-Py对Cu2+具有较好的选择性和灵敏性,在H2O/THF(V∶V=9∶1)溶液中对Cu2+显示出荧光淬灭效应,且不受其他金属离子干扰.细胞成像实验表明,探针TPE-Py可以检测细胞脂滴中的Cu2+.
Abstract
The fluorescent probe TPE-Py was designed and synthesized,and its structure was confirmed by NMR and HRMS.The recognition properties of probe TPE-Py for metal ions have been studied through fluorescence spectrophotometry and cell imaging experiments in HeLa cells.The results showed that TPE-Py had good selectivity and sensitivity for Cu2+.Among various metal ions,TPE-Py only showed exclusive fluorescence tum-off sensing on Cu2+in H2O/THF(V∶V=9∶1).The results of cell imaging experiments showed that the probe TPE-Py could detect Cu2+in lipid droplets of cells.
关键词
聚集诱导发光/荧光探针/离子识别/细胞成像Key words
aggregation-induced emission/fluorescent sensor/ion recognition/cell imaging引用本文复制引用
基金项目
国家自然科学基金(52173152)
国家自然科学基金(21805002)
陕西省科技创新团队(2022TD-36)
陕西省自然科学基金(2024JC-YBMS-123)
陕西省教育厅服务地方资助项目(23JC001)
出版年
2024
NETL
NSTL
万方数据