Inhibitory effects of different growth period Mogroside onα-glucosidase in vitro
Objective To study the inhibitory effects of different growth period Mogroside onα-glucosi-dase in vitro. Methods The Mogroside extract used in this study were extracted from Fructus Momordicae with fruit growth period of 30 days,50 days and 90 days.The Fructus Momordicae extract with good inhibition effect were screened out using reaction system with substrates ofα-glucosidase and PNPG.And inhibition effect of mogroside onα-glucosidase of Caco-2 cells was investigated. Results Mogroside had better inhi-bition onα-glucosidase activity,and its inhibitory effect onα-glucosidase activity had a significant positive correlation with dose.50-day mogroside extract had better inhibition onα-glucosidase than 30-day and 90-day mogroside extracts.The enzyme kinetics study results showed that inhibition effect of 50-day mogro-side onα-glucosidase belonged to mixed inhibition.50-day mogroside in dosage range from 0.05 to 50μg/ml tended to inhibit theα-glucosidase activity of Caco-2 cell after promoting.Mogroside at concentration of 5.00μg/ml and 10.00μg/ml could inhibitα-glucosidase activity in Caco-2 cells. Conclusion Inhibition effect of 50-day mogroside extract onα-glucosidase was greater than other growth period (30 d,90 d)mo-groside extract,inhibition onα-glucosidase activity in vitro depends on dosage and 50-day mogroside extract can also inhibitα-glucosidase activity of Caco-2 cells.
NETL
NSTL
万方数据
维普
