Objective This article aims to investigate the antioxidant activities of extracts from different parts of Nicotiana tabacum (N.tabacum). Methods The active constituents from the roots and leaves of N. tabacum were obtained by extracting with 90% alcohol and 50% alcohol as solvents.The antioxidant activities of different fractions to 1,1 - diphenyl - 2 - picrylhydrazyl radical (DPPH ·)were evaluated by using UV spectrophotometer. Results Whether the roots or the leaves,the antioxidant activity of their 50% ethanol fractions was better than other fractions,respectively.Among them,the best active fraction was the 50% eth-anol fractions from the roots,and its activity was close to the activities of vitamin C (Vc)and butylated hydroxytoluene (BHT)when its concentration reached or exceeded to 1 60 mg/L. Conclusion The constitu-ents of the roots and leaves in N.tabacum were different.In addition,the active constituents of anti-oxida-tion from N.tabacum were easily dissolved and eluted by strong polar solvents.
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