Effect of matrine on the proliferation,apoptosis and migration of lung cancer cell A549 and its mechanism
Objective To investigate the effect of matrine on the proliferation,apoptosis and migration of lung cancer A549 cells,as well as its mechanism.Methods A549 cells were treated with different concentra-tions of matrine.The growth inhibition of matrine against A549 cells was evaluated using the CCK-8 assay.The changes in apoptosis were analyzed using Hoechst 33528 combined with PI double staining.The ability of cell migration and invasion were analyzed using the scratch test and Transwell assay.The expressions of Opa1,p53,Bcl-2,Bax,Caspase-3,Cleaved-caspase-3,N-cadherin and Vimentin proteins were detected using West-ern Blot.Results Matrine could decrease the proliferation and increase the apoptosis rate of A549 cells.Ma-trine was found to be able to inhibit the invasion and metastasis of A549,as confirmed by the Transwell and scratch detection.The Western Blot analysis showed that matrine increased the expressions of p53,Bax and Cleaved-caspase-3 proteins in A549 cells.It also inhibited the expressions of Opa1,Bcl-2,Caspase-3,N-cad-herin,and Vimentin proteins in A549 cells.These changes exhibited a dose-effect relationship with the concen-tration of matrine.Conclusion Matrine can inhibit the proliferation,migration,and invasion of lung cancer cells while enhancing apoptosis.The pharmacological mechanism is related to the regulation of the Opa1/p53/Bcl-2/Bax/Caspase-3 signal axis,N-cadherin and Vimentin.
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