Effect of electroacupuncture on the expressions of Nrf2,HO-1 and PSD95 in the hippocampal CA3 region of diabetic rats
Objective To observe the effect of electroacupuncture(EA)on the expression changes of nu-clear factor E2 related factors(Nrf2),heme oxygenase-1(HO-1)and postsynaptic density protein 95(PSD95)in the hippocampal CA3 region of diabetic model rats,aiming to explore the possible mechanism of EA in pro-tecting the injury of diabetic nervous system.Methods Male Wistar rats were randomly divided into a nor-mal group,a vector group,a diabetic group and an EA group,with 8 rats in each group.The diabetic model was established by intraperitoneal injection of streptozotocin.One week later,the EA group received EA stim-ulation at the acupoints of"Zusanli"and"Yishu"for 30 min,once a day for 4 weeks.The fasting blood glucose level was measured by a glucose meter.The morphology of neurons in the hippocampal CA3 region was ob-served by Nissl staining.The protein expressions of Nrf2,HO-1 and PSD95 in the hippocampal CA3 region were detected by immunohistochemical staining.Results Compared with the normal group and the vector group,the diabetic group had increased blood glucose level(P＜0.05).Compared with the diabetic group,the EA group had decreased blood glucose level(P＜0.05).Compared with the normal group and the vector group,the diabetic group had reduced layers of neurons in the hippocampal CA3 region,with pyknosis in the nuclei Compared with the diabetic group,the EA group had increased layers of neurons in the hippocampal CA3 region,with improved morphology of neurons.Compared with the normal group and the vector group,the dia-betic group had decreased expressions of Nrf2,HO-1 and PSD95 in the hippocampal CA3 region(P＜0.05).Compared with the diabetic group,the EA group had increased expressions of Nrf2,HO-1 and PSD95 in the hippocampal CA3 region(P＜0.05),and the expression levels were similar to those of the normal group and the vector group(P＞0.05).Conclusion EA can up-regulate the expressions of Nrf2,HO-1 and PSD95 in the hippocampal CA3 region of diabetic model rats,suggesting that it may play a protective role on neurons by inhibiting oxidative stress and improving synaptic plasticity.
diabetes mellituselectroacupuncturenuclear factor E2 related factorsheme oxygenase-1postsynaptic density protein 95hippocampal CA3
万方数据
维普
