摘要
目的 研究槲皮素对急性酒精性胃溃疡小鼠的保护作用,并探讨其改善胃溃疡的潜在作用机制.方法 将60只雄性C57BL/6小鼠随机分为正常对照组、模型组、槲皮素低剂量组、槲皮素中剂量组、槲皮素高剂量组和奥美拉唑(OMZ)组.预灌胃给药7 d后,模型组和各给药组灌胃90％乙醇溶液建立急性胃溃疡模型.测定各组小鼠胃溃疡损伤指数、溃疡抑制率;采用酶联免疫吸附法(ELISA)检测血清白介素-6(IL-6)、肿瘤坏死因子α(TNF-α)、超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)的含量;HE染色法观察胃溃疡组织病理学变化;Western Blot检测Shh信号通路上Shh和Gli-1蛋白表达情况.结果 与正常对照组相比,模型组小鼠胃组织病理损伤加重;SOD、GSH的活力降低(P＜0.05),MDA的含量及血清IL-6和TNF-α的水平升高(P＜0.05),胃黏膜组织Shh和Gli-1蛋白表达下调(P＜0.05).与模型组相比,槲皮素和奥美拉唑组能改善酒精性胃溃疡的病理损伤;提高SOD、GSH的活力(P＜0.05),降低MDA的含量及血清IL-6和TNF-α的水平(P＜0.05),并增加胃黏膜组织Shh和Gli-1蛋白表达(P＜0.01).结论 槲皮素对酒精性胃溃疡具有一定的预防保护作用,其机制可能与调控Sonic Hedgehog信号通路抑制炎症反应和氧化应激有关.
Abstract
Objective To study the protective effects of quercetin on mice with acute alcoholic gastric ul-cers and explore its potential mechanisms in alleviating gastric ulcers.Methods Sixty male C57BL/6 mice were randomly divided into normal control group,model group,low-dose quercetin group,medium-dose quer-cetin group,high-dose quercetin group,and omeprazole(OMZ)group.After 7 days of pre-gavage,the model group and each treatment group were given the solution of 90％ ethanol to establish acute gastric ulcer models.The study measured gastric ulcer damage indexes and ulcer inhibition rates.Enzyme-linked immunosorbent as-say(ELISA)was adopted to assess the levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),super-oxide dismutase(SOD),malondialdehyde(MDA),and reduced glutathione(GSH)in serum.Histopathologi-cal changes in gastric ulcer tissues were observed using HE staining,and Western Blot was adopted to analyze the expressions of Shh and Gli-1 proteins in the Shh signaling pathway.Results Compared to the normal control group,the model group exhibited aggravated pathological injuries in gastric tissues.It showed de-creased activity of SOD and GSH(P＜0.05),increased MDA content,elevated levels of serum IL-6 and TNF-α(P＜0.05),and down-regulated expressions of Shh and Gli-1 proteins in gastric mucosa(P＜0.05).Compared with the model group,both quercetin and omeprazole groups demonstrated alleviation in pathological injuries associated with alcoholic gastric ulcers,with increased activity of SOD and GSH(P＜0.05),de-creased MDA content,reduced levels of serum IL-6 and TNF-α(P＜0.05),and elevated expressions of Shh and Gli-1 proteins in gastric mucosa(P＜0.01).Conclusion Quercetin exhibits a preventive protective effect against alcoholic gastric ulcers,and its mechanism may be associated with the regulation of the Sonic Hedgehog signaling pathway to suppress inflammatory responses and oxidative stress.
维普
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