Quercetin-mediated regulation of the Sonic Hedgehog signaling pathway and its protective effects in mice with acute alcoholic gastric ulcer
Objective To study the protective effects of quercetin on mice with acute alcoholic gastric ul-cers and explore its potential mechanisms in alleviating gastric ulcers.Methods Sixty male C57BL/6 mice were randomly divided into normal control group,model group,low-dose quercetin group,medium-dose quer-cetin group,high-dose quercetin group,and omeprazole(OMZ)group.After 7 days of pre-gavage,the model group and each treatment group were given the solution of 90％ ethanol to establish acute gastric ulcer models.The study measured gastric ulcer damage indexes and ulcer inhibition rates.Enzyme-linked immunosorbent as-say(ELISA)was adopted to assess the levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),super-oxide dismutase(SOD),malondialdehyde(MDA),and reduced glutathione(GSH)in serum.Histopathologi-cal changes in gastric ulcer tissues were observed using HE staining,and Western Blot was adopted to analyze the expressions of Shh and Gli-1 proteins in the Shh signaling pathway.Results Compared to the normal control group,the model group exhibited aggravated pathological injuries in gastric tissues.It showed de-creased activity of SOD and GSH(P＜0.05),increased MDA content,elevated levels of serum IL-6 and TNF-α(P＜0.05),and down-regulated expressions of Shh and Gli-1 proteins in gastric mucosa(P＜0.05).Compared with the model group,both quercetin and omeprazole groups demonstrated alleviation in pathological injuries associated with alcoholic gastric ulcers,with increased activity of SOD and GSH(P＜0.05),de-creased MDA content,reduced levels of serum IL-6 and TNF-α(P＜0.05),and elevated expressions of Shh and Gli-1 proteins in gastric mucosa(P＜0.01).Conclusion Quercetin exhibits a preventive protective effect against alcoholic gastric ulcers,and its mechanism may be associated with the regulation of the Sonic Hedgehog signaling pathway to suppress inflammatory responses and oxidative stress.
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