Mechanism study of sodium ferulate in improving learning and memory in vascular dementia rats via SIRT1/FOXO3A pathway
Objective To observe the neuroprotective effects of sodium ferulate on the prefrontal cortex of rats with vascular dementia(VD)and to explore its impact on the expression of SIRT1 and FOXO3A proteins.Methods Thirty-two male Sprague-Dawley(SD)rats were randomly divided into four groups,with 8 rats in each group.The model group and the sodium ferulate group underwent bilateral common carotid artery emboli-zation(BCCAO)to induce VD,the sham group underwent the same surgical procedure without ligation,and the normal group received no treatment.The sodium ferulate group was administered sodium ferulate solution(100 mg/kg)intragastrically for four weeks,while the other three groups received an equivalent volume of sa-line by gavage.The behavioral,cellular morphology,and protein expression levels of SIRT1 and FOXO3A were analyzed using the Morris water maze,Nissl staining,Western Blotting,and immunohistochemical stai-ning.Results Neurons in the normal and sham groups maintained intact morphological structures.In con-trast to the normal group,the model group demonstrated significantly prolonged escape latency,reduced plat-form crossings(P＜0.05),nuclear shrinkage,a significantly decreased expression of SIRT1(P＜0.05),and a notable increase in FOXO3A expression(P＜0.05).In comparison to the model group,the sodium ferulate group exhibited enhanced cortical cell morphology,decreased escape latency and FOXO3A protein expression(P＜0.05),and increased platform crossings and SIRT1 protein expression(P＜0.05).Conclusion Sodium ferulate enhances learning and memory abilities in VD rats by regulating SIRT1/FOXO3A pathway,providing a research foundation for the treatment of VD.
vascular dementiasilent information regulator 1forkhead box transcription factor O3ASodium ferulate
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