Objective To investigate the effects of stromal cell-derived factor-1(SDF-1)on the migration,collagen RNA expression and extracellular matrix expression of rat bone marrow mesenchymal stem cells(BM-SCs).Methods The effects of SDF-1 on BMSCs proliferation and migration were observed by CCK-8 assay and Transwell cell migration assay,respectively.The collagen RNA expression in BMSCs was detected by real-time fluorescence quantitative PCR.The collagen protein expression in BMSCs cell sheets was detected by Western blotting.The hyaluronic acid(HA)expression in BMSCs cell sheet culture medium was detected by enzyme-linked immunosorbent assay.Results The chemotactic effect of SDF-1 on BMSCs migration was concentration-dependent,while it had no significant effect on cell proliferation.SDF-1 decreased the ratio of RNA expression to protein expression in type Ⅰ collagen and type Ⅲ collagen,and significantly increased the HA expression level in BMSCs cell sheets(P＜0.05).Conclusion Within a suitable concentration range,SDF-1 can significantly promote BMSCs migration,reduce the ratio of RNA expression to protein expression in type Ⅰ collagen to type m collagen,and enhance HA expression levels.
维普
万方数据