ERβ promotes TGFBR1 expression,enhancing the proliferation and apoptosis in prostate cancer cells
Objective To explore how estrogen receptor P(ERβ)regulates Transforming Growth Factor Beta Receptor 1(TGFBR1)to promote the progression of prostate cancer.Methods Samples of prostate cancer patients were collected,and the expression of TGFBR1 was detected using immunohistochemistry.A si-lencing TGFBR1 vector was constructed to examine its effects on the proliferation and metastasis of prostate cancer.Various assays were employed,including cell cloning,CCK-8,EDU and apoptosis assays.A dual-lu-ciferase reporter gene assay was used to detect whether ERβ binds to TGFBR1.PCR and Western blot analyses were performed to assess the impact of ERβ interference on TGFBR1 expression.Results TGFBR1 was found to be overexpressed in prostate cancer tissue,with high expression levels directly related to poor progno-sis.Compared with the control group(NC),Cell cloning,CCK-8 and apoptosisassays showed that cell prolif-eration and apoptosis of TGFBR1 group were significantly inhibited,while cell proliferation and apoptosis of Oe-ER-β+siTGFBR1 group were not significantly different from those of NC group.Dual luciferase reporter gene assay revealed that ERβ binds to the TGFBR1 promoter sequence.Moreover,silencing ERβ led to a de-crease in TGFBR1 expression.Conclusion ERβ can promote the progression of prostate cancer by binding to TGFBR1 promoter sequence,thereby enhancing cell proliferation and apoptosis.
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