β-Solamarine induces apoptosis in breast cancer MCF-7 cells via the Fas-mediated death receptor pathway
Objective To investigate the apoptotic effects of β-solamarine(BSM)on breast cancer MCF-7 cells and its effect on proteins associated with the Fas-mediated death receptor pathway.Methods Different concentrations of BSM were used to interfere with breast cancer MCF-7 cells cultured in vitro.The cell prolif-eration inhibition rate was measured using MTT assay,while cell invasion assay was used to observe cell inva-sion ability.TUNEL assay was used to detect cell apoptosis.Western blot analysis was employed to detect the expression levels of Fas,FADD,Cleaved Caspase-8,Cleaved Caspase-3,Cleaved PARP,and PARP proteins.Results The cell group treated with BSM showed a higher cell inhibition rate,a significant decrease in the number of invasive cells and a significant increase in cell apoptosis rate,demonstrating statistically difference compared to the normal control group(P＜0.01).Treatment with the Caspase-8 inhibitor Z-IETD-FMK group significantly reduced apoptosis rate,demonstrating statistically difference(P＜0.01)compared to the BSM-treated group with the same dose.BSM treatment significantly upregulated the expression of Fas,FADD,Cleaved Caspase-8,Cleaved Caspase-3,and Cleaved PARP,while downregulating PARP expression in MCF-7 cells compared to the normal control group,demonstrating statistically difference.(P＜0.05 or P＜0.01).Conclusion BSM shows significant effects in inhibiting the proliferation,invasion and promoting ap-optosis in MCF-7 cells.The activation of the Fas-mediated death receptor pathway appears to be one of the mechanisms through which BSM induces apoptosis.
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