Expression and clinical significance of HMGA1 in peripheral blood and tissues of gastric cancer patients
Objective To investigate the expression and clinical significance of high mobility group AT-hook 1(HMGA1)in peripheral blood and tissues of gastric cancer patients.Methods Enzyme-linked immu-nosorbent assay and immunohistochemical staining were used to detect the expression of HMGA1 in peripheral blood and paraffin-embedded tissues of gastric cancer patients.Database analysis was searched to analyze the expression of HMGA1 in gastric cancer tissues and its relation to overall survival.Western Blot and qRT-PCR were used to detect the expression of HMGA1 protein and mRNA in tumor and adjacent tissues of gastric canc-er.Results HMGA1 expression in the peripheral blood of gastric cancer patients was higher than that of healthy medical examiners,with a statistically significant difference(P＜0.001),and the difference was sta-tistically significant with age of the patients,degree of differentiation,Lauren typing,degree of infiltration,and whether or not there were lymph node metastases(P＜0.001).HMGA1 expression was significantly higher on days 1,3,5,and 7 post-radical surgery compared to pre-surgery levels,and the difference was sta-tistically significant(P＜0.001).High expression of HMGA1 was associated with overall survival(OS)of gastric cancer,and increased HMGA1 protein and mRNA expression levels were observed in tumor tissues(P＜0.05).Conclusion There is certain valuable reference in HMGA1 expression detection from peripheral blood and tumor tissues of gastric cancer patients for clinical diagnosis and treatment.
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