The mechanism of RhoA/Cofilin signaling pathway in learning and memory and the expression of synaptic-related proteins in rats with chronic aluminum poisoning
Objective To investigate the regulatory effect of RhoA/Cofilin signaling pathway on synaptic-related protein expression and learning and memory function in hippocampus and cerebral cortex of rats with chronic aluminum poisoning.Methods A chronic aluminum poisoning model rat was established by intraper-itoneal injection of a 20 mmol/L maltol aluminum solution at a dose of 20 μmol/kg per day for 2 months,based on the daily body weight of the rats.The rat models of chronic aluminum poisoning were randomly divided into two groups:a model group and a RhoA inhibitor group.The RhoA inhibitor group received intraperitoneal in-jections of 40 mg/kg Rhosin hydrochloride once daily for 30 consecutive days.An additional blank control group was included,with 5 rats in each group.The Morris water maze positioning navigation experiment was used to record the swimming paths of the rats to find the platform.Western Blot analysis was performed to de-tect the expression levels of RhoA,Cofilin,synaptophysin(SYN),and postsynaptic density protein 95(PSD-95)in the hippocampus and cerebral cortex.Results The results of Morris water maze positioning navigation experiment showed that compared with the blank control group,the rats in the model group exhibited poorer trajectory orientation and deviated from the target platform.Compared with the model group,the movement trajectory of rats in the RhoA inhibitor group was more inclined to the target platform.Western Blot results showed that the relative expression levels of Cofilin,PSD-95,and SYN proteins in the hippocampal tissue and cerebral cortex of the aluminum poisoning model group were significantly lower than those of the blank control group(P＜0.05).The relative expression level of RhoA protein in the hippocampal tissue and cerebral cortex of the aluminum poisoning model group was significantly higher than that of the blank control group(P＜0.01).The relative expression levels of Cofilin,PSD-95,and SYN proteins in the hippocampal tissue and cere-bral cortex of the RhoA inhibitor group were significantly higher than those of the aluminum poisoning model group(P＜0.05).The relative expression level of RhoA protein in the hippocampal tissue and cerebral cortex of the RhoA inhibitor group was significantly lower than that of the aluminum poisoning model group(P＜0.05).Conclusion Inhibiting the RhoA/Cofilin signaling pathway can improve the learning and memory a-bilities of rats with chronic aluminum poisoning,which may be related to the up-regulation of the expression of RhoA,SYN,and PSD-95 proteins and the down-regulation of the expression of Cofilin protein in the hipp-ocampal tissue and cerebral cortex.
RhoA/Cofilin signaling pathwayaluminum maltolchronic aluminum poisoninglearning and memory abilitiessynaptic-related proteins
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