CWI1-2 protects ischemic stroke model by inhibiting NLRP3-mediated pyroptosis
Objective To investigate the protective effect of CWI1-2 in ischemic stroke models by inhibi-ting NLRP3-mediated pyroptosis.Methods m6A differential genes were screened using data from the GEO database.Animal models of ischemic stroke were established and divided into three groups:the NC group(normal control group),the cerebral ischemic stroke(CIS group)group,and the cerebral ischemic stroke+[insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2)inhibitor]CWI1-2group(CIS+CWI1-2 group).TTC and HE staining were performed to assess the modeling of each group.Immunofluorescence was utilized to detect the expression intensities of NeuN,TUNEL,and NLRP3.Western blot(WB)analysis was conducted to examine the expression levels of NLRP3,Caspase-1,IL-1β,and GSDMD in each group.Results Bioinformatics results indicated that IGF2BP2 was highly expressed in patients with CIS.Animal model re-sults showed that the infarction area in the CIS group was significantly increased compared to the NC group,whereas the infarction area in the CIS+CWI1-2 group was decreased compared to the CIS group.HE staining revealed increased nuclear fragmentation in the CIS group,with partial nuclear recovery observed in the CIS+CWI1-2 group.Immunofluorescence results indicated that compared to the NC group,the CIS group exhibited decreased NeuN expression,increased NLRP3 expression,and heightened TUNEL fluorescence intensity.However,in the CIS+CWI1-2 group,NeuN expression was increased,NLRP3 expression was decreased,and TUNEL fluorescence intensity was reduced compared to the CIS group.WB results demonstrated elevated pro-tein expression levels of NLRP3,Caspase-1,IL-1β,and GSDMD in the CIS group compared to the NC group,with decreased expression levels of these proteins observed in the CIS+CWI1-2 group compared to the CIS group.Conclusion CWI1-2 exerts a protective effect in the ischemic stroke model by inhibiting NLRP3-me-diated pyroptosis.
insulin-like growth factor 2 mRNA-binding protein 2 inhibitorNLR family,the thermal protein domain contains protein 3stroke
万方数据
维普
