Study on the effect of Undaria pinnatifida polysaccharide on mice with type 2 diabetes mellitus combined with non-alcoholic fatty liver disease
Objective To investigate the effect of Undaria pinnatifida polysaccharide(PUP)on mice with type 2 diabetes mellitus(T2DM)combined with non-alcoholic fatty liver disease(NAFLD).Methods Fifty healthy 4-week-old male Kunming mice were fed a high-fat and high-sugar diet for 3 weeks.In the second week,the mice were fasted for 12 hours with free access to water,and 5 mice were randomly selected for blood collection from the ocular globe to measure blood lipid levels.Significant differences in blood lipid levels com-pared to a normal group were observed.Subsequently,the mice were fasted for 12 hours and then intraperito-neally injected with streptozotocin at a dose of 0.075 g/kg for 3 consecutive days.Mice with blood glucose lev-els of 11.1 mmol/L or above were considered to have successfully developed the model.The mice were divided into 6 groups:normal control group,metformin group,PUP high-dose,medium-dose,and low-dose groups,and T2DM combined with NAFLD group.The drug-administered groups were gavaged with PUP at doses of 0.8 g/kg,0.4 g/kg,and 0.2 g/kg,respectively,and metformin at a dose of 0.5 g/kg.Three days later,fast-ing and 2-hour postprandial blood glucose levels,as well as fasting TG,TC,LDL-C,HDL-C,ALT,and AST levels were measured and histopathological changes in the liver tissue of each group were examined.Results Fasting blood glucose results:Compared with the T2DM combined with NAFLD group,the blood glucose con-tent in each experimental group was decreased,and the difference was statistically significant(P＜0.05).However,for 2-hour postprandial blood glucose,no statistically significant differences were observed between the experimental groups and the T2DM combined with NAFLD group,except for the normal group.Lipid re-sults:There were no significant differences in TG levels among the groups(P＞0.05).TC and HDL-C levels were significantly reduced in all experimental groups compared to the T2DM combined with NAFLD group(P＜0.01).LDL-C levels showed significant differences between the metformin group,and the T2DM combined with NAFLD group(P＜0.05).Transaminase results:ALT levels showed significant differences between each experimental group and the T2DM combined with NAFLD group(P＜0.05).AST levels only showed significant differences between the metformin group and the T2DM combined with NAFLD group(P＜0.05).Conclusion PUP has certain hypoglycemic and hypolipidemic effects on mice with T2DM combined with NAFLD,with the high-dose group showing the best effect.Its hypolipidemic effect is mainly targeted at TC and HDL-C.The effect of reducing transaminase levels is consistent with the results of HE staining of liver tis-sue.Therefore,PUP has a certain therapeutic effect on mice with T2DM combined with NAFLD.
万方数据
维普
