Effect of Remimazolam on Th17 cells and their related factors in septic mice
Objective To investigate the effect of remimazolam on Th17 cells and their related factors in septic mice induced by cecal ligation.Methods Thirty-two male C57BL/6 mice were divided into 4 groups:a blank control group(Group N),a remimazolam group(Group R),a sepsis group(Group S),and a sepsis+remimazolam group(Group S+R).Mice were sacrificed by orbital blood collection 24 hours after injection.The pathological scores of lung tissues were observed under a light microscope after staining with hematoxylin-eosin(HE)method.The wet/dry(W/D)ratio of lung injury in mice was detected.The concentrations of IL-17 in plasma and lung tissues were detected by ELISA.The mRNA of RORγt in lung tissues was detected by real-time fluorescence quantitative PCR.The proportion of Th17 cells among CD4+T cells in peripheral blood and lung tissues was detected by flow cytometry.Results Obvious lung injury occurred in mice 24 hours af-ter cecal ligation.Compared with Group N,the scores of lung tissues and the W/D ratio were significantly in-creased,the expression levels of IL-17 in plasma and lung tissues of mice were increased,the expression of RORγt mRNA in lung tissues was increased,and the proportion of Th17 cells in CD4+T cells in blood and lung tissues was increased(P＜0.05).After preoperative remimazolam pretreatment,compared with Group S,lung injury in Group S+R was significantly improved,the scores of lung tissues and the W/D ratio were sig-nificantly decreased compared with Group N,the expression levels of IL-17 in plasma and lung tissues of mice were decreased,the expression of RORγt mRNA in lung tissues was decreased,and the proportion of Th17 cells in CD4+T cells in blood and lung tissues was decreased(P＜0.05).Conclusion Pretreatment with remimazolam can alleviate the degree of septic lung injury in mice,and its mechanism may be related to reduc-ing the differentiation of Th17 cells and decreasing the expression of IL-17 and its transcription factor RORγt.
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维普
