Preliminary exploration of the association between KRT19 and favorable prognosis in gastric cancer patients based on single-cell sequencing
Objective To initially explore the value of keratin 19(KRT19)as a biomarker for gastric cancer progression and prognostic diagnosis using single-cell sequencing technology-driven bioinformatics tech-niques.Methods The TCGA database was used to download gastric cancer datasets for differential gene ex-pression analysis,ROC analysis,immune infiltration analysis,GSEA enrichment analysis,and drug sensitivity analysis.Single-cell sequencing data of gastric cancer samples and normal gastric tissue samples collected from the GEO database were subjected to batch correction,normalization,dimensionality reduction,clustering,an-notation,pseudotime analysis,infercnv malignant prediction analysis,and CellChat analysis.Venn diagrams were drawn using the jvenn plotting website.Genes including KRT19 were subjected to KM survival curve plotting using the KM plotter website.Protein-protein interaction analysis of the KRT19 gene was performed using the string website.Results KRT19 was highly expressed in gastric cancer tissues(P＜0.001),par-ticularly in the epithelial cells of these tissues.High KRT19 expression was associated with favorable prognosis in gastric cancer patients(P＜0.05).High-expressing KRT19 epithelial cells emerged at the late stage of gas-tric cancer differentiation.KRT19 primarily influenced interactions between gastric cancer epithelial cells through the MDK(MK)signaling pathway.High-expressing KRT19 epithelial cells were benign.KRT19 ex-pression positively correlated with neutrophil and plasma cell infiltration and negatively correlated with CD8+T-cell infiltration.KRT19 interacted with genes such as KRT18 and AFP.KRT19 functioned in pathways such as keratinization and ribosomal small subunit biogenesis.High KRT19 expression exhibited lower drug sensi-tivity to drugs like 5-fluorouracil and ethylene glycol compared to low KRT19 expression.Conclusion KRT19 may be highly expressed in late-stage differentiated gastric cancer epithelial cells,promoting the ap-pearance or proliferation of benign epithelial cells in gastric tissue and contributing to a favorable prognosis in gastric cancer development.
万方数据
维普
