Determination of Magnesium Isotopic Composition of Plant Chlorophylls Based on HPLC and MC-ICP-MS
The accurate determination of magnesium isotope values of chlorophyll a and chlorophyll b is particularly important for the study of magnesium biosynthesis pathways during chlorophyll formation.HPLC is required before MC-ICP-MS can be utilized,but HPLC lacks a method for separating chlorophylls.Therefore,it is necessary to develop a set of methods for separating plant chlorophyll a and chlorophyll b.The samples separated by this method are suitable for magnesium isotope determination by MC-ICP-MS.Based on the detection wavelength of 665nm and C18 column(7.6mm×250mm,5μm),the HPLC conditions were optimized by three-factor and three-level orthogonal design to obtain the required parameters.The correlation coefficients of chlorophyll a and chlorophyll b standard curves in the concentration range of 5-50mg/L were greater than 0.9996,the detection limits were 0.40-1.09mg/L,the quantification limits were 1.22-3.31mg/L,the relative standard deviation was less than 8.10％,and the recoveries were 91.92％-111.11％.The magnesium isotope data of the isolated samples also showed that the column temperature of 25℃,flow rate of 1mL/min,and methanol-acetone(80∶20,V/V)mobile phase were reliable as HPLC separation conditions.The method established in this paper provides technical support for the separation of chlorophyll a and chlorophyll b in plants,and the separated samples can be used for magnesium isotope determination.The BRIEF REPORT is available for this paper at http://www.ykcs.ac.cn/en/article/doi/10.15898/j.ykcs.202208300161.
magnesium isotopestandard-sample-bracketing methodhigh performance liquid chromatographymobile phasechlorophyll achlorophyll b
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维普
