摘要
目的:建立离子色谱/电导检测法测定百合中二氧化硫残留量的分析方法.方法:试样经酸蒸馏提取,离子色谱法测定硫酸根离子含量.选用 IonPac AS19 阴离子交换分离柱,在线产生 40mmol/L KOH 淋洗液等度洗脱,流速 1.0mL/min,以抑制型电导检测器检测.结果:在 0.0μg/mL～200.0μg/mL 浓度范围内,线性关系良好,检出限为 2.26mg/kg,定量限为 7.53mg/kg,回收率为 91.52%～98.65%(RSD 值为 0.89%～1.54%),精密度为 0.40%～0.57%.结论:该方法操作简便、批量处理时间短、定性定量准确、灵敏度高,适合百合中二氧化硫残留量的检测.
Abstract
Object:A new method was established to determine residual sulfur dioxide in Lilium brownii by ion chromatography with conducting detection.Methods:Samples were extracted by acid distillation.The separation was performed on an Dionex IonPac AS19 anion analytical column.The mobile phase consisted of 40 mmol/L KOH solution in isocratic elution at a flow rate of 1.0mL/min.Auto-generated conducting detection ASRS ULTRA II 4mm was used.Results:The results showed that the linear relation of residual sulfur dioxide was good in the range of 0.0～200μg/mL(r=0.9998).The limits of detection of the method was 2.26mg/kg and the limit of quantitation was 7.53mg/kg.The average recoveries of residual sulfur dioxide in Lilium browniiranged between 91.52%～98.65%at different spiked levels with relative standard deviation from 0.89%to 1.54%.Precision was from 0.40 to 0.57%.Conclusion:The convenient operation and versatility of this method is suitable for the fast screening and detection of residual sulfur dioxide in Lilium brownii.
维普
万方数据