Application of Comparative Teaching Method in Experimental Teaching of Large-scale Instruments-Take the Determination of Carbofuran in Tomato Juice as an Example
The comparative teaching method was applied in the experimental teaching of High Performance Liquid Chromotography(HPLC)and High Performance Liquid Chromotography-Mass Spectrometry(HPLC-MS)by taking the determination of carbofuran in tomato juice as an example.In the theoretical teaching part,the similarities and differences of the discipline,structure,application in the determination of carbofuran between HPLC and HPLC-MS and their determination of carbofuran were compared.In the practical operation part,different results were given by HPLC and HPLC-MS since the carbofuran content in tomato juice was lower than the detection limit of detection of HPLC method but higher than that of HPLC-MS method.The teaching efficiency and the comprehensive application ability of students for large-scale instruments were increased substantially by the comparative teaching method,which is meaningful to the application-oriented talent cultivation in colleges and universities.