液相色谱法同时检测浓缩苹果汁中乳酸、富马酸和羟甲基糠醛

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中文摘要：建立了同时检测果汁中的乳酸、富马酸和羟甲基糠醛的高效液相色谱分离方法.采用 YMC-pack ODS-AQ 色谱柱(4.6mm×250mm,5μm),流动相为 0.03%磷酸、2%甲醇水溶液,流速为 0.4 mL/min～0.8mL/min,柱温为35℃.实验结果表明,乳酸和富马酸在 210nm 处、羟甲基糠醛在 280nm 处得到有效分离,相关系数分别为0.99951、0.99939 和 0.99999.回收率分别为 95.97%、98.54%和 96.50%.该方法被成功地用于同时定量测定浓缩苹果汁中的乳酸、富马酸和羟甲基糠醛.

外文标题：Simultaneous Determination of Lactic Acid,Fumaric Acid and Hydroxymethyl Furfural in Concentrated Apple Juice by Liquid Chromatography

外文摘要：A HPLC method for simultaneous determination of lactic acid,fumaric acid and hydroxymethyl furfural in fruit juice was developed.The chromatography was performed on an YMC-pack ODS-AQ column(4.6mm×250mm,5μm)with mobile phase consisting of 0.03%phosphoric acid and 2%methanol solution.The flow rate was 0.4mL/min～0.8mL/min and the column temperature was 35℃.The results showed that lactic acid and fumaric acid were effectively separated at 210nm and hydroxymethyl furfural at 280nm,and the correlation coefficients were 0.99951、0.99939 and 0.99999,respectively.The recoveries were 95.97%、98.54%and 96.50%,respectively.The method has been successfully used for simultaneous quantitative determination of lactic acid,fumaric acid and hydroxymethyl furfural in concentrated apple juice.

外文关键词：

liquid chromatographylactic acidfumaric acidhydroxymethyl furfural

作者：

辛刚、党敏花

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作者单位：

国投中鲁果汁股份有限公司,北京 100037

关键词：

液相色谱法乳酸富马酸羟甲基糠醛

出版年：

2024

饮料工业

中国饮料工业协会

饮料工业

影响因子：0.315

ISSN：1007-7871

年,卷(期)：2024.27(4)