Purpose:To study the development of isotonic electrolyte beverage.Method:Through the single factor experiment,the influence factors of osmotic pressure and the gradient of osmotic pressure were investigated,and the formula was designed by osmotic pressure value and sensory evaluation,so as to develop the isotonic electrolyte beverage.Result:Dietary glucose added amount:4.2%,crystalline trehalose added amount:1.7%,sea salt added amount:0.03%,potassium chloride added amount:0.02%,isotonic electrolyte beverage tasted best.The osmotic pressure was 307mOsmol/L,which met the requirements of isotonic food.Conclusion:Sugar and electrolyte were the raw materials of osmotic pressure.According to the test process formula optimization,the product taste was the best.