A high performance liquid chromatographic(HPLC)method was developed for the determination of formaldehyde in milk and dairy products.The test samples were derivatized with 2,4-dinitrophenylhydrazine solution-sodium acetate buffer solution(pH=5),extracted by sonication for 10min,placed in a constant temperature shaker at 60℃and shaken at 150 r/min for 1 h to convert the formaldehyde to the corresponding stable formaldehyde-2,4-dinitrophenylhydrazone.The generated product was separated on a C18 column with the mobile phase of acetonitrile-water(60∶40,V∶V),detected by UV detector at 365 nm detection wavelength,and quantified by external standard method.The results showed that formaldehyde had a good linear relationship in the concentration range of 0 μg/mL-5 μg/mL with a correlation coefficient of 0.999.The relative standard deviations(RSDs)of formaldehyde in milk powder,liquid milk and yogurt at the three spiked levels were 0.46%～2.67%,0.91%～3.86%and 1.93%～3.11%,respectively,and the recoveries of formaldehyde in milk powder,liquid milk and yogurt at the three spiked levels were 90.2%～91.7%,98.5%～101%and 98.0%～101%,respectively.The test method is simple,rapid,accurate and reproducible,and provides a method for the determination of formaldehyde content in milk and dairy products.
关键词
高效液相色谱法/乳与乳制品/甲醛
Key words
high performance liquid chromatography/milk and dairy products/formaldehyde
维普
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