Objective:To develop a high performance liquid chromatography-mass spectrometry(HPLC-QDa)method for the determination of arabinose,fructose,glucose,sucrose,maltose,erythritol,sorbitol and mannitol in drink and juice.Methods:Sample was extracted by vortex oscillated,ultrasonic extracted and diluted with extraction solution,then analyzed by high performance liquid chromatography-mass spectrometry(HPLC-QDa).The mobile phase A was 90%acetonitrile-5%water-5%isopropanol(V/V/V).The mobile phase B was 80%acetonitrile-20%water(V/V).The gradient elution was carried at a flow rate of 0.85mL/min and the column temperature at 65℃,and the sample injection volume was 1μL.Sugar and sugar alcohol were qualitatively determined by retention time and selected ions,and were quantified by external standard method.Results:The linear relationship of arabinose,fructose,glucose,sucrose,maltose,erythritol,sorbitol and mannitol was good(R2>0.99)in the concentration range of 2-100μg/mL.The spiked recoveries of drink are within the range of 94.40%~111.94%.The relative standard deviation(RSD)of method precision was 0.68%~4.62%(n=6).And the spiked recoveries of juice are within the range of 90.51%~109.94%.The relative standard deviation(RSD)of method precision is 0.71%~4.82%(n=6).Conclusion:This method is applicable for the determination of arabinose,fructose,glucose,sucrose,maltose,erythritol,sorbitol and mannitol in drink.