摘要
目的:建立了一种高效液相色谱-质谱法检测饮料中阿拉伯糖、果糖、葡萄糖、蔗糖、麦芽糖、赤藓糖醇、山梨糖醇和甘露糖醇含量的方法.方法:样品经涡旋混匀,超声提取和稀释后,经高效液相色谱串联质谱分析.流动相为A相(90%乙腈+5%水+5%异丙醇),B相(80%乙腈+20%水),梯度洗脱,流速为 0.85mL/min,柱温为 65℃,样品进样量为 1μL,以保留时间选择离子定性,以外标法定量.结果:阿拉伯糖、果糖、葡萄糖、蔗糖、麦芽糖、赤藓糖醇、山梨糖醇和甘露糖醇在 2μg/mL～100μg/mL质量浓度范围之间具有良好的线性关系(R2>0.99);饮料的加标回收率在 94.40%～111.94%;方法精密度相对标准偏差 RSD%(n=6)在 0.68%～4.62%.果汁的加标回收率在90.51%～109.94%;方法精密度相对标准偏差RSD%(n=6)在 0.71%～4.82%.结论:本方法可应用于饮料中阿拉伯糖、果糖、葡萄糖、蔗糖、麦芽糖、赤藓糖醇、山梨糖醇和甘露糖醇的测定.
Abstract
Objective:To develop a high performance liquid chromatography-mass spectrometry(HPLC-QDa)method for the determination of arabinose,fructose,glucose,sucrose,maltose,erythritol,sorbitol and mannitol in drink and juice.Methods:Sample was extracted by vortex oscillated,ultrasonic extracted and diluted with extraction solution,then analyzed by high performance liquid chromatography-mass spectrometry(HPLC-QDa).The mobile phase A was 90%acetonitrile-5%water-5%isopropanol(V/V/V).The mobile phase B was 80%acetonitrile-20%water(V/V).The gradient elution was carried at a flow rate of 0.85mL/min and the column temperature at 65℃,and the sample injection volume was 1μL.Sugar and sugar alcohol were qualitatively determined by retention time and selected ions,and were quantified by external standard method.Results:The linear relationship of arabinose,fructose,glucose,sucrose,maltose,erythritol,sorbitol and mannitol was good(R2>0.99)in the concentration range of 2-100μg/mL.The spiked recoveries of drink are within the range of 94.40%～111.94%.The relative standard deviation(RSD)of method precision was 0.68%～4.62%(n=6).And the spiked recoveries of juice are within the range of 90.51%～109.94%.The relative standard deviation(RSD)of method precision is 0.71%～4.82%(n=6).Conclusion:This method is applicable for the determination of arabinose,fructose,glucose,sucrose,maltose,erythritol,sorbitol and mannitol in drink.
NETL
NSTL
万方数据