Research on the Application of Microbial Method Based on National Standard Optimization in the Detection of Vitamin B12 Content in Beverages
Vitamin B12 is essential for maintaining normal physiological functions in the human body,but it is found in beverages in low quantities,and traditional detection methods are limited in beverages.In order to solve this problem,the third method based on GB 5009.285-2022"National Food Safety Standard-Determination of Vitamin B12 in Food"was used to optimize the preparation,extraction and dilution process of the sample.The representativeness and experimental accuracy of the sample were improved by mixing pre-packaged liquid beverages,introducing ultrasonic waves to remove CO2 and crushing solid beverages.According to the difference in vitamin B12 content,the extraction and dilution strategies were flexibly.The samples of highconcentration were treated following theGB method,and the samples of lowconcentration were treated by simplified method.The results showed that the optimized method could accurately determine the content of vitamin B12 in beverages,and the relative standard deviations of the samples were less than 15%,which were controlled within 4.93%,1.87%,4.07%,and 5.40%.The recovery rate of spiked sample was stable between 77%～08%,which meets the requirements of 60%～120%spiked recovery,with good repeatability,accuracy and reliability.It was consistent with the test results of third-party institutions,showing good practicability and economy,and providing a new way for the detection of vitamin B12 in beverages.
vitamin B12health productoptimizationnational standard microbiology law
NETL
NSTL
万方数据
