Study on the Determination of Lipase Activity in Milk by p-Nitrobenzene Palmitate Method
A method for the determination of the lipase activity was established by using p-nitrophenyl palmitate as a substrate to determine the product p-nitrophenol at 405 nm UV absorption wavelengths.In the study,the effects of experimental conditions such as the optimum pH of lipase hydrolysis,the amount of substrate,the ratio of sample to buffer and incubation time on the detection results were explored.The results show that under the optimal experimental conditions,the correlation of the curve was 0.9964,the recovery rate was 64%～69%,the precision was 5.47%～6.72%,and the relative standard deviation was 4.83%.Through the comparison of two kinds of lipase detection kits,the samples with different lipase content were detected,and it was found that the trend of lipase activity was the same,indicating that this method was accurate,simple,rapid and suitable for the detection of lipase activity in dairy products.This method is expected to provide an alternative detection method for quality control in milk.
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