高效液相色谱法测定食品中透明质酸钠的含量
Determination of Sodium Hyaluronate Content in Food by High Performance Liquid Chromatography
杨静 1刘春丽 1黎俊威 1胡杰 1廖宇琼1
作者信息
- 1. 捷通(广州)检测技术服务有限公司,广东 广州 510730
- 折叠
摘要
目的:建立反向液相色谱法测定食品中透明质酸钠的分析方法.方法:含透明质酸钠的样品,根据基质的不同,可采用直接提取法或醇沉提取法,提取完的样品溶液经过与透明质酸酶反应,再用高效液相色谱仪分析.色谱柱为 MCI GEL CK08EH(8mm×300mm,9μm),流动相:1%磷酸溶液,流速为 0.6mL/min,柱温为 40℃,进样体积20μL,检测波长 232nm,外标法定量.结果:透明质酸钠的相关系数为 1.0000,加标回收率为 93.2%～100.3%,相对偏差为 2.71%和 3.26%,检出限为 0.3mg/kg,定量限为 1.0mg/kg.结论:该方法适用范围广,准确度、精密度良好,适用于食品中透明质酸钠含量的测定.
Abstract
Purpose:To establish the method for determining the content of sodium hyaluronate in food by reverse liquid chromatography.Methods:For pretreatment,direct extraction or alcohol precipitation extraction could be used depending on the substrate.An MCI GEL CK08EH column(8mm×300mm,9µm)was used with 1%phosphoric acid as mobile phase.The flow rate was 0.6mL/min,the column temperature was 40℃,and the injection volume was 20µL.The detection wavelength was 232nm.Results:The correlation coefficient of sodium hyaluronate was 1.0000,the spiked recovery rate was 93.2～100.3%,and the relative deviation was 2.71%和 3.26%.The detection limit was 0.3mg/kg,and the quantification limit was 1.0 mg/kg.Conclusions:This method shows a wide range of applicability,good accuracy and precision,and is suitable for the determination of sodium hyaluronate content in food.
关键词
高效液相色谱法(HPLC)/透明质酸钠/食品Key words
high performance liquid chromatography(HPLC)/sodium hyaluronate/food引用本文复制引用
出版年
2024
NETL
NSTL
万方数据