Method for Characterizing the Porous Structure of Absorbable Collagen Materials
Objective To develop a method for characterizing the microstructure of absorbable collagen materials,and analyze their porosity and pore size.Methods Field emission scanning electron microscope(SEM)was used,images of non-gold-sprayed samples and gold-sprayed samples were captured at different acceleration voltages.The obtained SEM images were analyzed for porosity and pore size by using Image J software,and the availability of the results was verified.Results The clear SEM images were obtained by using a lower acceleration voltage for non-gold-sprayed samples.After gold spraying,the ability to withstand voltage was significantly improved,and the clear SEM images could be obtained even at higher acceleration voltages.The obtained SEM images were analyzed for pore size and porosity using Image J software.Among them,the average porosity was 50.9%and the average pore size was 11.9 μm.The dispersion coefficient of porosity was 8.6%.and the dispersion coefficient of pore size was 6.7%.Conclusion The combination of field emission SEM and Image J software is an effective method for characterizing the porous structure of absorbable collagen materials,which can solve the issue of missing methods in relevant standards.
field emission scanning electron microscopeabsorbable collagenporous structureImage J software
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