Expression Levels and Efficacy Evaluation of miRNA-27a and TOP2A Protein in Breast Cancer Chemotherapy
Objective To analyze the expression levels and predictive efficacy of microRNA-27a(miRNA-27a)and DNA topoisomerase Ⅱ-Alpha(TOP2A)protein in breast cancer chemotherapy.Methods The medical records of 47 patients with breast cancer admitted to the Breast and Thyroid Surgery Department of Liaocheng People's Hospital from January 2020 to January 2022 were retrospectively analyzed,and all patients received chemotherapy treatment.The expression level of miRNA-27a in plasma was detected by quantitative real-time PCR method,and the expression of TOP2A protein was detected by immunohistochemistry method.Differences in the expression levels of miRNA-27a and TOP2A protein before and after chemotherapy were compared,and the relationship between miRNA-27a and TOP2A protein and poor prognosis of breast cancer chemotherapy was analyzed by Logistic regression analysis.Receiver operating characteristic(ROC)curve was drawn to analyze the application value of miRNA-27a and TOP2A proteins in the prediction of chemotherapy efficacy for breast cancer.Results The levels of miRNA-27a and TOP2A protein detected in 47 patients at the fourth month after starting chemotherapy were significantly lower than before chemotherapy(upon admission)(P<0.05).At the fourth month after starting chemotherapy,35 cases(74.47% )had a good prognosis,while 12 cases(25.53% )had a poor prognosis,and the detection values of miRNA-27a and TOP2A protein in the good prognosis group were lower than those in the poor prognosis group.Logistic regression analysis showed that high expression of miRNA-27a and TOP2A protein was a risk factor for poor prognosis in breast cancer chemotherapy(P<0.05).The ROC curve showed that the combination of miRNA-27a and TOP2A protein had the largest area under curve(0.875,95% CI:0.798-0.952)in predicting poor prognosis of breast cancer chemotherapy,with sensitivity and specificity of 91.67% and 94.29%,respectively(P<0.05).Conclusion miRNA-27a and TOP2A proteins are highly expressed in patients with poor prognosis of breast cancer chemotherapy.If the levels increased significantly,it indicates a potential risk of disease deterioration and limited efficacy of chemotherapy.
breast cancerchemotherapymiRNATOP2A proteinhuman epidermal growth factor receptor 2
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