Effects of CacyBP/SIP on Invasion,Migration and Angiogenesis of Stromal Cells in Endometriosis
Objective To explore the mechanism of Calcyclin-binding protein/Siah-1-interacting protein(CacyBP/SIP)on the invasion,migration,and angiogenesis of endometriosis(EMs)stromal cells.Methods A total of 60 patients with EMs who visited the Obstetrics Department of Shijiazhuang Maternal and Child Health Hospital from January 2021 to December 2022 were selected as the research subjects.Through laparoscopic surgery,endometrial tissue was taken and endometrial stromal cells were isolated.The recombinant CacyBP/SIP lentivirus and inhibitor transfection was used to detect the virus and inhibit the transfection results.The experiment was divided into 5 groups including CacyBP/SIP lentivirus group,lentivirus negative control group,CacyBP/SIP inhibitor group,inhibitor negative control group,and untreated group.Quantitative PCR was used to identify CacyBP/SIP levels in each group of cells,Transwell method was used to detect cell invasion and migration in each group,MTT colorimetric method was used to detect the proliferation of cells in each group,flow cytometry was used to detect cell apoptosis in each group,immunoblotting was used to detect the expression of vascular endothelial growth factor(VEGF)and cyclooxygenase-2(COX-2)in cells of each group.Results Compared with the inhibitor negative control group,the CacyBP/SIP lentivirus group showed an increase in cell proliferation at 24,48 and 72 h(P<0.05);however,compared with the CacyBP/SIP lentivirus group,the CacyBP/SIP inhibitor group showed a decrease in cell proliferation(P<0.05).Compared with the inhibitor negative control group,the CacyBP/SIP lentivirus group showed an increase in CacyBP/SIP levels,number of cell invasion,migration,and expression of VEGF and COX-2 proteins(P<0.05);however,compared with the CacyBP/SIP lentivirus group,the CacyBP/SIP inhibitor group showed a decrease in above indicators(P<0.05).The cell apoptosis rates in the untreated group,lentivirus negative control group,inhibitor negative control group,CacyBP/SIP lentivirus group,and CacyBP/SIP inhibitor group were 8.54%±0.26%,8.42%±0.38%,8.46%±0.19%,3.38%±0.15%,and 18.42%±1.23%,respectively.Compared with the inhibitor negative control group,the cell apoptosis rate in the CacyBP/SIP lentivirus group decreased(P<0.05);compared with the CacyBP/SIP lentivirus group,the apoptosis rate of cells in the CacyBP/SIP inhibitor group increased(P<0.05).Conclusion The low expression of CacyBP/SIP can reduce the proliferation,invasion and migration ability of EMs mesenchymal cells,which is speculated to inhibit angiogenesis by inhibiting VEGF and COX-2 genes.
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