Effect of Silencing Rictor on Angiogenesis,Tumorigenesis and P4HB/Hedgehog Expression of Hepatocellular Carcinoma Cells in Vitro
Objective To investigate the effect of silencing Rictor on angiogenesis,tumorigenesis and expression of P4HB/Hedgehog in hepatocellular carcinoma cells in vitro.Methods The hepatocellular carcinoma cells were collected and divided into liver cancer cell group(LC group),liver cancer cell+Rictor-NC group(NC group)and liver cancer cell+siRictor group(SR group).The transfection of each group was observed under fluorescence microscope,and the expression of Rictor was detected by real-time PCR.The ability of the cells to form mimetic vessels was observed by three-dimensional culture,and the tumor-forming ability was observed by tumor-forming assay in nude mice.The angiogenesis related indicators and the expression of P4HB/Hedgehog were detected by western blotting.Results There was no transfection in LC group,but green fluorescence was seen in NC group and SR group,indicating successful transfection;and the transfection rate was above 90%,indicating that the transfected cells were stable.The expression of Rictor mRNA,the number of angiogenesis,tumor growth curve,vascular endothelial growthfactor,P4HB and Hedgehog protein in the SR group were significantly decreased(P<0.05),indicating that Rictor silence can promote apoptosis of hepatocellular carcinoma cells and inhibit tumor formation.Conclusion Silencing Rictor can significantly inhibit the angiogenesis of hepatocellular carcinoma cells in vitro,reduce tumorigenesis in nude mice,and inhibit the expression of P4HB/Hedgehog.
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维普
