心脏常温机械灌注装置的研制
Development of Heart Normothermic Machine Perfusion Device
刘倩 1刘雯雁 2赵长缨 1闫炀 1王飞扬 1郑幸龙1
作者信息
- 1. 西安交通大学第一附属医院心血管外科,陕西 西安 710061
- 2. 西安交通大学第一附属医院血液净化科,陕西 西安 710061
- 折叠
摘要
目的 研制一种心脏常温机械灌注装置,以实现移植心脏的离体保存.方法 设备利用蠕动泵和膜式氧合器模拟人工心肺,通过给离体心脏顺行灌注常温含血灌注液,实现移植心脏体外节律搏动及长时间保存.同时通过灌注液乳酸水平判断心脏灌注状态,实时监测心脏灌注流量及压力.结果 本设备的重要参数可基本满足需求,且已经在中华小型猪心脏(n=3)上进行离体灌注实验.结果显示心脏离体后搏动良好,初步验证了其可行性.结论 常温机械灌注为心脏移植器官保存提供了新思路,且有望延长心脏离体保存时间,扩大潜在供体器官数量.
Abstract
Objective To introduce a heart normothermic machine perfusion device to achieve in vitro preservation of transplanted hearts.Methods The peristaltic pumps and membrane oxygenators were utilized to simulate an artificial heart and lung.The solution of infusing normothermic blood perfusate to the transplanted heart was used to achieve rhythmic beating and long-term preservation of the transplanted heart in vitro.At the same time,The cardiac perfusion status was determined by the lactate level of the perfusate,and the cardiac perfusion flow and pressure were monitored in real-time.Results The device parameters met the necessary requirements,and it had been used for in vitro perfusion experiments in Chinese miniature porcine hearts(n=3).The results showed that the heart was well paced in vitro,which preliminary validated its feasibility.Conclusion Normothermic machine perfusion provides a novel strategy for heart preservation and is expected to extend the preservation time of the heart in vitro and expand the number of potential donor organs.
关键词
心脏保存/常温机械灌注/离体灌注/心脏移植/设备研发/器官评估Key words
heart preservation/normothermic machine perfusion/in vitro perfusion/heart transplantation/equipment research/organ evaluation引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据