Development of Heart Normothermic Machine Perfusion Device
Objective To introduce a heart normothermic machine perfusion device to achieve in vitro preservation of transplanted hearts.Methods The peristaltic pumps and membrane oxygenators were utilized to simulate an artificial heart and lung.The solution of infusing normothermic blood perfusate to the transplanted heart was used to achieve rhythmic beating and long-term preservation of the transplanted heart in vitro.At the same time,The cardiac perfusion status was determined by the lactate level of the perfusate,and the cardiac perfusion flow and pressure were monitored in real-time.Results The device parameters met the necessary requirements,and it had been used for in vitro perfusion experiments in Chinese miniature porcine hearts(n=3).The results showed that the heart was well paced in vitro,which preliminary validated its feasibility.Conclusion Normothermic machine perfusion provides a novel strategy for heart preservation and is expected to extend the preservation time of the heart in vitro and expand the number of potential donor organs.
国家科技期刊平台
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