Study on the Method of Detecting the Circle of Willis in the Neonatal Mouse Brain by 3D Vascular Doppler Ultrasound with Experimental Small Animal Ultrasound Instrument
Objective To explore the method of detecting the circle of Willis in the neonatal mouse brain by three-dimensional(3D)vascular ultrasound technology of small animals.Methods The neonatal mice with post-natal ages of 1 d(n=6)and 7 d(n=6)underwent ultrasound scan imaging using the ultra-high resolution ultrasonic imaging system at a center frequency of 30 MHz,and the detection path and direction of the circle of Willis were determined.Doppler imaging and 3D vascular ultrasound of the entire mouse brain with non-invasively through the intact skull and skin were performed to display the circle of Willis in brain.At the same time,the blood flow signals of the middle cerebral artery(MCA)of newborn mice were detected,and the end diastolic velocity(EDV),peak systolic velocity(PSV),resistive index(RI)and coefficient of variation(CV)were measured.The morphology and structure of the circle of Willis in neonatal mice were observed by perfusion technique.Results With the anterior fontanelle as the detection path,the probe was continuously scanned downward at a 45° angle,and color Doppler vascular imaging combined with 3D reconstruction technology was used to display the circle of Willis of newborn mice in stereo,which was consistent with the morphology and structure of the circle of Willis shown by perfusion technique.Pulse Doppler could be used to collect stable MCA blood flow signals,CV was 1.9%～7.9%,and there were no statistical significances in EDV,PSV and RI of MCA blood flow signals on the left and right sides(P>0.05).Conclusion It is feasible to non-invasively display the structure of the circle of Willis in neonatal mouse by 3D vascular ultrasound using small animal ultrasound system,and to detect stable blood flow parameters of MCA.This study can provide an experimental basis for understanding the structure and function of the circle of Willis in living mice during postnatal development.
ultra-high-frequency ultrasound3D vascular ultrasoundcircle of Willismiddle cerebral artery(MCA)neonatal micehemodynamics
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