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混合测序技术在高通量测序中的应用

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介绍了高通量测序在面向大样本时所面临的问题,分析了高通量测序技术的原理和特点及其在解决大样本测序时的技术优势,并总结了检测矩阵的构建方法,指出了混合测序能够应用于部分高通量测序实验并可大幅节约测序成本,展望了混合测序技术在高通量测序中的应用前景.
Application of pooled sequencing technology to high-throughout sequencing
The problems of high-throughout sequencing technology are introduced when used for large samples,whose principle,characteristics and advantages are also analyzed.The construction of the test matrix is summarized.It's pointed out that the introduction of pooled sequencing into some high-throughout sequencing experiments may result in decreased cost.The prospect of pooled sequencing technology is explored in the high-throughout sequencing.

composite samplehigh-throughout sequencinggroup testingdisjunct matrix

李成

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210002南京,南京军区联勤部药品仪器检验所

样本混合 高通量测序 群试 分离矩阵

2014

医疗卫生装备
军事医学科学院卫生装备研究所

医疗卫生装备

CSTPCD
影响因子:0.776
ISSN:1003-8868
年,卷(期):2014.35(9)
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