Gene cloning and phylogenetic analysis on the Gα from nematode trapping fungus Drechslerella dactyloides
Dgal,a gene encoding Got,was firstly cloned from nematode - trapping fungus Drechslerella dac-tyloides by using methods of degenerate PCR and DNA Walking. The Dgal comprised 1 402 base pairs in length, and encoded a polypeptide with 356 amino acid residues. The theoretic molecular mass and pl of Dgal are deduced as 40. 84 ku and 6.68,respectively. A phylogenetic analysis on fungal Got indicates that the Dgal belongs to group Ⅲ of fungal Got, which possibly has the function as stimulating the adenylyl cyclases.
NETL
NSTL
万方数据
维普
