摘要
综合采用正相、反相硅胶柱色谱、Sephadex LH-20 凝胶柱色谱、半制备液相色谱等色谱方法进行化学成分分离纯化,运用核磁共振和质谱等波谱方法鉴定化合物的结构,对药用植物狗牙根[Cynodon dactylon(L.)Pers.]95%乙醇提取物进行化学成分研究,并对其中的黄酮类和蒽醌类进行抗炎活性研究.从狗牙根全草中分离得到 16个化合物,分别鉴定为苜蓿素(1)、chrysoeriol(2)、isoliquiritigenin(3)、tricin 4′-O-(threo-β-guaiacyl-(7″-O-methyl-9″-O-acetyl)-glyceryl)ether(4)、木犀草素(5)、tricin 4′-O-(erythro-β-guaiacylglyceryl)ether(6)、calquiquelignan D(7)、calquiquelignan E(8)、5,5′-dihydroxy-2′,4′-dim-ethoxyflavone-7-O-β-D-(6″-O-Z-p-coumaroyl)-glucopyranoside(9)、4′-hydroxy-2,3-dihydroflavone-7-β-D-glucopyranoside(10)、tricin 4′-O-(threo-β-guaiacyl-(7″-O-methyl)-glyceryl)ether(11)、ω-Hydroxyemodin(12)、phomarin(13)、aurantio-obtusin(14)、β-谷甾醇(15)、豆甾醇(16).除化合物 5 外,其他化合物均为首次从该植物中分离得到,其中化合物 2、3和 14 显示显著抗炎活性,其IC50 值分别为2.74、2.35、3.61 μmol/L.
Abstract
The chemical constituents of the 95％ethanol extract from Cynodon dactylon(L.)Pers.were studied using silica gel,open ODS,Sephadex LH-20,and semi preparative HPLC column chromatography.The anti-inflammatory activities of isolated flavonoids and anthraquinones were studied.Sixteen compounds were isolated from bermudagrass and identified as tricin(1),chrysoeriol(2),isoliquiritigenin(3),tricin4′-O-(threo-β-guaiacyl-(7″-O-methyl-9″-O-acetyl)-glyceryl)ether(4),luteolin(5),tricin 4′-O-(erythro-β-guaiacylglyceryl)ether(6),calquiquelignan D(7),calquiquelignan E(8),5,5′-dihydroxy-2′,4′-dim-ethoxyflavone-7-O-β-D-(6″-O-Z-p-coumaroyl)-glucopyranoside(9),4′-hydroxy-2,3-dihydroflavone-7-β-D-glucopyranoside(10),tricin 4′-O-(threo-β-guaiacyl-(7″-O-methyl)-glyceryl)ether(11),ω-Hydroxyemodin(12),phomarin(13),aurantio-obtusin(14),β-sitosterol(15)and stigmasterol(16).Compounds 1,2,3,4,6,7,8,9,10,11,12,13,14,15 and 16 were isolated from this plant for the first time.Compounds 2,3 and 14 showed significant anti-inflammatory activity,with IC50 values of 2.74,2.35 μmol/L and 3.61 μmol/L.
基金项目
云南省创新团队项目(202005AE160004)
云南省科技厅-昆明医科大学基础研究重点项目(202201AY070001-004)
维普
万方数据