Black rot,caused by Fusarium oxysporum,is a significant disease affecting the production and cultivation of Echeveria succulents.To manage this disease,we isolated and identified an endophytic Bacillus subtilis strain,OLR3-9,from the roots of wild rice(Oryza longistaminata).The strain's biocontrol efficacy was evaluated through in vitro antifungal activity,greenhouse trials,and colonization assessments.Results demonstrated that OLR3-9 effectively controls black rot in two Echeveria varieties,'Romeo Rubin'(E.agavoides)and'Jing Ye'(E.derenbergii),with mycelial inhibition rates of 63.45%and 66.60%,spore inhibition rates of 78.74%and 76.45%,and pot trial control effects of 65.55%and 73.13%,respectively.Interaction with OLR3-9 resulted in mycelial deformation and reduced sporulation of the pathogen,alongside stable colonization of the host plant's roots,stems,and leaves,thereby preventing pathogen invasion and spread.The Bacillus subtilis strain OLR3-9 shows strong potential as a biocontrol agent against black rot in Echeveria succulents.
Bacillus subtilisblack rot disease on succulent plantFusarium oxysporumEcheveriabiological control
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