露水草中β-蜕皮甾酮提取工艺优化及其体外抗氧化能力研究
Optimization of Extraction Process of β-ecdysterone from Cyanotis Arachnoidea and Study on its Antioxidant Capacity in Vitro
张彦昕 1柴贝贝 1石玉节 1李海利2
作者信息
- 1. 郑州赛科药业科技有限公司,河南 郑州 450000
- 2. 河南省农业科学院畜牧兽医研究所,河南 郑州 450000
- 折叠
摘要
为探究露水草β-蜕皮甾酮的最佳提取工艺,分别考察提取方式和提取温度对 β-蜕皮甾酮含量的影响;使用高效液相色谱法检测含量,利用DPPH和ABTS方法评价最佳提取工艺得到 β-蜕皮甾酮的抗氧化能力.使用温浸提取和加热回流两种提取方式,分别考察了60、80、100℃下溶剂为水的提取物中 β-蜕皮甾酮的含量;选择最佳提取工艺进行提取,进行方法学考察;利用DPPH和ABTS体外自由基清除实验测定其抗氧化活性.结果表明,以水为溶剂,100℃加热回流的提取方式得到的 β-蜕皮甾酮的含量最高;在该液相条件下,露水草样品可快速检出并且峰型良好,分离度高.通过自由基清除实验表明其具有体外抗氧化能力并呈一定浓度依赖型.
Abstract
To investigate the optimal extraction technology of β-ecdysterone from Cyanotis arachnoidea,the effects of extraction method and ex-traction temperature on the content of β-ecdysterone were investigated,the content was detected by HPLC,and the antioxidant capacity of β-ecdysterone obtained by the optimal extraction technology was evaluated by DPPH and ABTS methods.The content of β-ecdysterone in extracts with water as solvent at 60℃,80℃ and 100℃ were investigated by warm soaking extraction and heated reflux extraction,and the best extrac-tion technology was selected to carry out methodological investigation.The antioxidant activity of DPPH and ABTS was determined by free radi-cal scavenging experiments in vitro.The extraction technology study showed that the content of β-ecdysterone was the highest when water was used as the solvent and reflux was heated at 100℃.The methodological results showed that under the liquid phase condition,the samples could be quickly detected with good peak shape and high separation degree.The free radical scavenging experiments showed that the antioxidant activ-ity was concentration-dependent in vitro.
关键词
露水草/β-蜕皮甾酮/高效液相色谱/方法学验证/抗氧化Key words
cyanotis arachnoidea/β-ecdysterone/high performance liquid chromatography/methodology validation/antioxidant引用本文复制引用
基金项目
河南省科技攻关计划(182102410038)
郑州市高端外国专家技术合作项目(YZ22JSHZ021)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据