GC Determination of Pyrazole Boronic Acid Pinacol Ester and Validation of Analytical Method
Objective:The GC method was established for the detection of pyrazole boronic acid pinacol ester and validation of analytical meth-od.Method:The 5％phenyl-methyl polysiloxane(30 m×φ0.25 mm,0.25 μm)column was used;The detector was FID and the carrier gas was nitrogen with a flow rate of 1.0 mL/min;The injection temperature was 215℃;The detection temperature was 295℃;The split ratio was 3:1;The injection volume was 0.1 μL;The initial column temperature was 50℃and maintained for 2 min,the column temperature was raised to 150℃ at the rate of 10℃/min and then maintained for 0 min,the column temperature was raised to 260℃ at the rate of 30℃/min and then maintained for 5 min;The H2 flow rate was 30 mL/min;The air flow rate was 300mL/min;The N2 flow rate was 25 mL/min.Result:The results of specificity,repeatability,linearity,intermediate precision and solution stability all meet the requirements.Conclusion:This method is fast and accurate that can be used for the determination of related substance of pyrazole boronic acid pinacol ester.
GC methodpyrazole boronic acid pinacol estervalidation of analytical method
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NSTL
万方数据
维普
